We narrowed to 871 results for: lic
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TypeBlog Post...Janssen Award dinner which took place at the New York Public library. Being in the presence of scientific heroines...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...
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CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...tools available for bacteria and some of the applications for which they’ve been used. The beginnings ... E. coli. Furthermore, to demonstrate wider applicability they demonstrated success of Cas9 mediated repression...as a collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases...scenario allows for several potential CRISPR applications. First, CRISPR can be used to target locations... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...: Find sequences for plasmids assembled from publications and commercially available sources, as well ...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer... -
Hot Plasmids - October 2022
TypeBlog Post...+ over other Na+ and other cations. B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer 2/3 ...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more... -
Year of the Camelid: Antibody Style
TypeBlog Post...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative... excitement around using sdAbs in diagnostic applications. For example, fluorescently and radioactively...inspired this versatile family of tools, the applications they have already enabled, and the discoveries... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...should be. He debated taking a position as an application support/sales representative in industry or a...communicator than a science writer these days. I do some public speaking and a bit of video work for webinars (...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post... Specifically, viruses are able to infect and replicate in host cells, but they are susceptible to cells... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site. The... -
Choosing a Good Mentor for Scientists
TypeBlog Post... writing, lab budgeting, equipment acquisition, public speaking and personnel management? If you are ...ask a lot of questions. Personality and style conflicts are a common reason for scientists to change labs...websites listing alumi and it is easy to use publication records, university websites, ResearchGate and... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post... at a high multiplicity of infection, because this setup allows for experimental replicates for each of...rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell library construction... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post... be used as a fusion tag for a wide range of applications from traditional imaging to FRET and stochastic...fluorescent protein: structural characterization and applications in fluorescence imaging." Biochemical Journal...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents... -
Another Pathway into Cells: iTOP
TypeBlog Post...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing. Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References... -
K. phaffii: Rising to the Occasion
TypeBlog Post...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... Glucose Glucose, methanol, glycerol Heterothallic mating Efficient Efficient Inefficient... ancient yeast strains and metazoan cells. Applications for K. phaffii There’s a number of different... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...their multiplicity of infection so that a single TF was delivered to each cell, future applications could...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Biology of the NationalAcademy of Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior... -
DAP arrays for multiplex gene editing
TypeBlog Post...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening. Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...playing a card game called “Magic”. There were complicated rules that governed how this game was played.... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced... cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...improve our shipment processes. After reading a publication describing how short term storage on dry ice ...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...exposed to CO2/dry ice. All samples were tested in duplicate. What we found Not all tubes (or rather, not ...