We narrowed to 478 results for: mag

1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...introduce all desired codon changes (step 1 in the image above). A second universal primer is used to synthesize...

2. Five Popular Model Organisms

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   Blog Post

   ...Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis...

3. Plasmids 101: Biotinylation

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   Blog Post

   ...PMCID: PMC307283. 13. Howarth, M., & Ting, A. Y. “Imaging proteins in live mammalian cells with biotin ligase...

4. Antibodies 101: Beyond Surface Labeling

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   Blog Post

   ...negatively selecting your cells of interest is magnetic bead-activated cell sorting, or MACS. But MACS...

5. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ... the voltage setting of the laser impacts the magnitude of FSC, SSC, and all fluorescent stains. The cytometer's...

6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...interactions between these proteins is hard to imagine yet alone study. To elucidate these interactions...

7. Evolution of Lab Techniques

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   Blog Post

   ...restriction enzymes that cut DNA at specific sites (5). Imagine that you have a genome with unknown sequence. In...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...diseases, including dementia, gut disorders, nerve damage, and cardiac disease. This increased disease risk...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   ...For a simple example of using Gibson assembly, imagine that you want to insert your gene of interest into...

10. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ... and the information is often confidential (see image below). Slide of information gathered by Postdoctoral...

11. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ... your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery will need...

12. Fluorescence Titering Assay

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    Protocol

    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

13. Protocol - Bacterial Transformation

    Type

    Protocol

    ...cause the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce...

14. Exploring Applications of the Bioluminescent HiBiT Tag

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    Blog Post

    .... Assessing phototoxicity in live fluorescence imaging. Nature Methods, 14(7), 657–661. https://doi.org...

15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ... AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic...

16. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

    ...asked a question, I felt like I was yelling. I imagined 6 years of biting my tongue and gratefully moved...

17. Finding Your Perfect Job After University

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    Blog Post

    ...me because it was sufficient to figure out if my image of lab work lived up to reality, and, if it is wasn...

18. Colony Formation Titering Assay

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    Protocol

    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

19. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ...orientation. T-LR= transposons left-right orientation. Image from Leo Vo.   TL;DR Transposons are another...

20. Finding Your Science Policy Path

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    Blog Post

    ...many ways to prepare for a science policy career. Image used with permission from SCI. Many thanks to...