We narrowed to 560 results for: mme

1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...do the same for you. Send us your questions or comments to [email protected]. We're eager to ...

2. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ... are standard but can take seven to 10 days. Commercially available kits provide titers in just two days...

3. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification...

4. Antibodies 101: Flow Compensation

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   Blog Post

   ...starting to use flow cytometry, it’s generally recommended to start compensating with single-color controls...

5. Plasmids 101: Degron Tags

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   ...Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem...

6. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...manually in water baths but is now easily done in a programmed thermocycler. For more details on this procedure...

7. Hot Plasmids: Winter 2025

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   Blog Post

   ...antibodies targeting the human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT...

8. Common Injection Routes in Mice

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   Blog Post

   ... mice are under anesthesia, but they are not recommended for tumorigenic compounds, as circulation is ...

9. Experimenting with New Careers while in Grad School

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   Blog Post

   ...organization. My short experience with my blog helped immensely when CINSO started brainstorming the resources...

10. Genome-wide Screening Using CRISPR

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    Blog Post

    ...compared to the rest of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence...

11. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ... a backbone as a single unit. Although it is recommended to use the 2A peptides instead of an IRES when...

12. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...function of the FP-fusion protein. Therefore, it is recommended to use monomeric FPs (usually denoted by a “m...

13. AAVs in Retinal Gene Therapy

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    Blog Post

    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...

14. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ... to answer some of their great questions, was immensely gratifying. I came out of the experience with ...

15. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter...

16. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...and ovarian lines commonly used in lab. It is recommended you check your cell lines for HR-related mutations...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...potential instabilities in the China data, so we recommend interpreting the model results for China with ...

18. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...recombinant monoclonal antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing...

19. Great Results Start with Great Standard Curves

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    Blog Post

    ...involving sigmoidal curves, manufacturers often recommend using advanced graphing software designed to plot...

20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated...