We narrowed to 565 results for: mme

1. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...manually in water baths but is now easily done in a programmed thermocycler. For more details on this procedure...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...

3. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...making targeted modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. ...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...let us know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved...

5. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...questions during the talk, jot them down for the Q&A immediately after. For one conference I went to, I made it...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...do the same for you. Send us your questions or comments to [email protected]. We're eager to ...

7. Hot Plasmids: Winter 2025

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   Blog Post

   ...antibodies targeting the human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT...

8. Common Injection Routes in Mice

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   Blog Post

   ... mice are under anesthesia, but they are not recommended for tumorigenic compounds, as circulation is ...

9. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ... are standard but can take seven to 10 days. Commercially available kits provide titers in just two days...

10. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification...

11. Antibodies 101: Flow Compensation

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    Blog Post

    ...starting to use flow cytometry, it’s generally recommended to start compensating with single-color controls...

12. Plasmids 101: Degron Tags

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    Blog Post

    ...Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem...

13. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...family-run business – 3D Molecular Designs – to commercialize some of the best instructional materials that...

14. Experimenting with New Careers while in Grad School

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    Blog Post

    ...organization. My short experience with my blog helped immensely when CINSO started brainstorming the resources...

15. Genome-wide Screening Using CRISPR

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    Blog Post

    ...compared to the rest of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence...

16. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ... a backbone as a single unit. Although it is recommended to use the 2A peptides instead of an IRES when...

17. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...function of the FP-fusion protein. Therefore, it is recommended to use monomeric FPs (usually denoted by a “m...

18. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification...

19. AAVs in Retinal Gene Therapy

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    Blog Post

    ...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...

20. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...potential instabilities in the China data, so we recommend interpreting the model results for China with ...