Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 760 results for: mpo

Showing: 441 - 460 of 760 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...fragment, any additional recombination events are impossible even in the presence of Cre recombinase. This...in the absence of the endogenous gene. Spatio-temporal expression of optogenetic reporters: Since most...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...expression levels, the terminator also plays an important role in RNA processing and contributes to variability...polyadenylation. The addition of the poly(A) tail is important for stability of the mRNA, protection from degradation...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...quantified using qPCR primers targeting specific viral components such as LTRs, gag, WPRE, antibiotic resistance-genes...genomic DNA isolated, and primers targetting viral components or the transgene itself are used to quantify ...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too...you won't get as much out of it and it will be impossible to schedule. In the pre-event interviews, the...

  5. Of Myc and Men

    Type
    Blog Post
    ... for every potential new target and basically impossible if you wanted to distinguish between endogenous... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

  6. PhD Applications After COVID

    Type
    Blog Post
    ...course of the last few years: recognizing the importance and benefit of remote and video communication...staff have become a new way to allow you to get important information about the campus even if you can’t...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...proteins are assembled in vitro using purified components of the transcription and translation machinery...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...

  11. Common Injection Routes in Mice

    Type
    Blog Post
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...biomedical applications of these tools difficult or impossible. But if you’ve ever had an MRI, you know that...the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could be expressed...

  14. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been...

  16. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...mentor and graduate office. I cannot stress how important they were to my success as a student. They didn...and more voices of encouragement emphasize the important roles of scientists outside of academia One recent...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...

  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein...In both cases, the lipid membrane is the only component that is not dependent on viral genes. It is derived...
Showing: 441 - 460 of 760 results