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  2. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...mentor and graduate office. I cannot stress how important they were to my success as a student. They didn...and more voices of encouragement emphasize the important roles of scientists outside of academia One recent...

  3. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...proteins are assembled in vitro using purified components of the transcription and translation machinery...

  5. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been...

  7. Of Myc and Men

    Type
    Blog Post
    ... for every potential new target and basically impossible if you wanted to distinguish between endogenous... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

  8. PhD Applications After COVID

    Type
    Blog Post
    ...course of the last few years: recognizing the importance and benefit of remote and video communication...staff have become a new way to allow you to get important information about the campus even if you can’t...

  9. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...

  10. Common Injection Routes in Mice

    Type
    Blog Post
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...biomedical applications of these tools difficult or impossible. But if you’ve ever had an MRI, you know that...the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could be expressed...

  15. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

  16. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...that can be monitored non-invasively over time. Importantly, it is an immune privileged compartment, meaning...underway and reported results are encouraging. Importantly, a significant amount of basic research is being...

  19. MXS Chaining

    Type
    Blog Post
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...
Showing: 441 - 460 of 750 results