We narrowed to 976 results for: nin

...have used PCR-based screening to detect mutants, but this method limits screening to a few hundred worms...In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for...background. Their new method uses a selection cassette containing genes for hygromycin resistance and the Roller...

...Science Careers has developed a web-based, career-planning platform called the Individual Development Plan...have to be ‘extra’ Today’s graduate and postdoc training focuses on using multidisciplinary, collaborative...incorporating EAs into your routine.  Getting started: Planning your career path and identifying needed skills...suit these skills and interests: Use career planning tools such as the Individual Development Plan ...

... If you are in this position, you should start planning for your visa and or green card process well before...about the process early, keeping good records, and planning for the route to a rewarding career. It is more...this post, we discuss the following paths to maintaining a status in the United States including green... for self-sponsorship of the I-140 application meaning that the category does not require a foreign national... your current PI and Ph.D. or Masters PI. The remaining letters should be from independent people – i....

... in our repository. Instead, we facilitate the signing of MTAs by the Provider and Recipient (in Addgene...under which your material will be distributed. In signing this agreement, your organization agrees to let... a form needs to be signed. But the truth is, meaningful progress depends on things being organized, tracked... be used. Setting clear expectations from the beginning makes the sharing process smoother, more consistent...

...Antibodies administered as drugs are still immunogenic, meaning that they elicit an immune response from the body...the safety and the efficacy.  Better together Combining in silico and in vitro approaches enables researchers...method of immunogenicity assessment can help in determining the T-cell epitopes that will likely bring about...silico Immunogenicity Assessment for Sequences Containing Unnatural Amino Acids: A Method Using Existing...

...obligate aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon... that it predominantly uses non-homologous end joining (NHEJ) to perform DNA double strand break repair...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

...) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to... library can be used with other selection and screening mechanisms, although the unique barcodes for each...mutation and/or TF dysregulation. A targeted TF screening approach can not only lead to a better understanding...Cas-9 knockout and transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot...

...prevented. Keep this information in mind if you’re planning on shipping something on dry ice or if you’re ...they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours of CO2...it back in the freezer? We then tested whether returning the tubes to the -80°C freezer after storage on...Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following ...

...result in a blue, green, or red readout. But gRNAs containing two different hairpins will produce a secondary... depending on the hairpin combination. A gRNA containing all three hairpins produces a white color. While...simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin sequence (sgRNA-Sirius-8XPP7)...CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating MS2 and PP7 sequences (sgRNA-Sirius...

...Addgene also distributes pooled plasmid libraries containing hundreds, thousands, or even a million plasmids...plasmid. However, lots of library DNA is needed for screening experiments, so the scale of the DNA prep is larger...where each transformant forms its own colony, uninfluenced by the growth of other cells. For libraries ...wishes you luck in your future library use and screening, and if you have any tips, input, or stories of...

...Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus...their target sequence of choice (see tips for designing a gRNA) and their prefered Cas9 variant (with ...bacterial genomes are transcribed into pre-crRNAs containing both the spacer region and the direct repeat ...thinking about the types of experiments you're planning on doing and read the eBook or browse our CRISPR...

...everything that could be done with RNAi in terms of determining what genes are involved in biological process...the real hits is so much shorter…. it’s a great screening technology.” Doench thinks that the future of...become more efficient in terms of editing and screening) such that thousands of individual edits can be...says he’s “known of Addgene basically since the beginning and [has] been using Addgene ever since” and that...

...of the University of British Columbia. When transitioning from an academic science career path to a non-academic... lectures? These transferable skills could be meaningful for your potential employer. For example, my ... Developing Transferable Skills During Science Training and So You Think You have Skills.   Get Science...graduate students in job searching. Start your career planning early, start now Don't wait until the day you ...

... translation (Noren et al., 1989). In the late nineties, this was developed into an E. coli system, with...will be needed to introduce the gene of interest containing the chosen codon that will result in the incorporation... parameters the same for all three components, meaning that the final protocol may involve a sensitive... located at Oregon State University focused on turning GCE tools developed in the literature into robust...

...introduce frameshift mutations in genes of interest, turning expression off permanently. Viral vectors can ... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity...

...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...research. The EU, for example, is calling for the opening of sources within the next few years, with the ...

...interest. Isotype controls are used to make sure the staining you see is due to target-specific antibody binding...binding rather than nonspecific background staining. Isotype controls are used in antibody-based applications...switch to expressing a different isotype, the intervening DNA sequence is deleted, which places the variable...domain gene cluster. DNA recombination can delete intervening heavy-chain constant domain genes, placing the...

...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...antibodies will exhibit off-target binding to unintended proteins. This is because monoclonal antibodies...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use...plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some of the most requested... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

...are available for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli...Addgene. A set of mammalian expression vectors containing various spike protein variants are also available...received Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...