...that my piecemeal degree might one day foster a meaningful day job only came into focus when an English ...podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation, graphic design...interested in connecting with the public in a meaningful way, and not solely to ensure a profit. Often...Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro to Our Summer SciComm Series!...

...the transgene will be lost. Over time, bacteria containing the recombined vector backbone will also outgrow...samples. For a more detailed comparison of these cloning strains, browse the table of common gene mutations...there are both large and small colonies. Bacteria containing the recombined product tend to grow faster, so...N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia...

...when you’re running your next AAV experiment, use these questions as a guide for designing your future...Data Hub to quickly find details they need for planning their next experiments using a specific AAV tool...activate receptors? If you’re in the process of designing your experiment, read our blog post on important...

...constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists...fused them to sequences called complementarity determining regions (CDRs, or loops) of an antibody that ...bound to HA epitopes for minutes in living cells, meaning that scientists can track the movement and localization... & Givol, D. (1972). Localization of antibody-combining sites within the variable portions of heavy and...

...instruction. Six of the kits, known collectively as Greening the Classroom, were developed and tested by ABRC...such as instructional videos and datasheets. The remaining kits were donated to ABRC by the Arabidopsis research...has many benefits. Engaging students in active learning through hands-on experiments allows them to develop... out opportunities to engage people in science learning. Whether visiting classrooms, hosting field trips...

...daily basis, many found these tools inadequate for planning, visualizing, and documenting their procedures...procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer... even if one or two codons are missing at the beginning or end of the feature. With the increasing popularity...people love to use, and likes to start at the beginning, shepherding a new product from the idea stage...

...to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial ...part of interpersonal communication, which makes honing your ability to tell stories a similarly crucial... with me. First I calmed the situation down by opening a dialogue specifically about the project and contributions...

...acknowledged perks of scientific and technical training is that these educational paths prepare you for...perspective on other types of science careers as their training progresses.  In From Academic Solos to Industrial...explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless jump into...quickly Jack/Jane of all trades More sources of training and resources within the company Must find own...

...have used PCR-based screening to detect mutants, but this method limits screening to a few hundred worms...In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for...background. Their new method uses a selection cassette containing genes for hygromycin resistance and the Roller...

...Science Careers has developed a web-based, career-planning platform called the Individual Development Plan...have to be ‘extra’ Today’s graduate and postdoc training focuses on using multidisciplinary, collaborative...incorporating EAs into your routine.  Getting started: Planning your career path and identifying needed skills...suit these skills and interests: Use career planning tools such as the Individual Development Plan ...

... If you are in this position, you should start planning for your visa and or green card process well before...about the process early, keeping good records, and planning for the route to a rewarding career. It is more...this post, we discuss the following paths to maintaining a status in the United States including green... for self-sponsorship of the I-140 application meaning that the category does not require a foreign national... your current PI and Ph.D. or Masters PI. The remaining letters should be from independent people – i....

... in our repository. Instead, we facilitate the signing of MTAs by the Provider and Recipient (in Addgene...under which your material will be distributed. In signing this agreement, your organization agrees to let... a form needs to be signed. But the truth is, meaningful progress depends on things being organized, tracked... be used. Setting clear expectations from the beginning makes the sharing process smoother, more consistent...

...obligate aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon... that it predominantly uses non-homologous end joining (NHEJ) to perform DNA double strand break repair...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

...result in a blue, green, or red readout. But gRNAs containing two different hairpins will produce a secondary... depending on the hairpin combination. A gRNA containing all three hairpins produces a white color. While...simultaneously, Ma and Pederson created another sgRNA containing the PP7 hairpin sequence (sgRNA-Sirius-8XPP7)...CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating MS2 and PP7 sequences (sgRNA-Sirius...

...) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to... library can be used with other selection and screening mechanisms, although the unique barcodes for each...mutation and/or TF dysregulation. A targeted TF screening approach can not only lead to a better understanding...Cas-9 knockout and transcriptional activation screening. Nat Protoc. 2017 (4):828-863. doi: 10.1038/nprot...

...Antibodies administered as drugs are still immunogenic, meaning that they elicit an immune response from the body...the safety and the efficacy.  Better together Combining in silico and in vitro approaches enables researchers...method of immunogenicity assessment can help in determining the T-cell epitopes that will likely bring about...silico Immunogenicity Assessment for Sequences Containing Unnatural Amino Acids: A Method Using Existing...

...Addgene also distributes pooled plasmid libraries containing hundreds, thousands, or even a million plasmids...plasmid. However, lots of library DNA is needed for screening experiments, so the scale of the DNA prep is larger...where each transformant forms its own colony, uninfluenced by the growth of other cells. For libraries ...wishes you luck in your future library use and screening, and if you have any tips, input, or stories of...

...prevented. Keep this information in mind if you’re planning on shipping something on dry ice or if you’re ...they would be exposed to a closed environment containing CO2 (from the dry ice). After 48 hours of CO2...it back in the freezer? We then tested whether returning the tubes to the -80°C freezer after storage on...Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following ...

...Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus...their target sequence of choice (see tips for designing a gRNA) and their prefered Cas9 variant (with ...bacterial genomes are transcribed into pre-crRNAs containing both the spacer region and the direct repeat ...thinking about the types of experiments you're planning on doing and read the eBook or browse our CRISPR...

...everything that could be done with RNAi in terms of determining what genes are involved in biological process...the real hits is so much shorter…. it’s a great screening technology.” Doench thinks that the future of...become more efficient in terms of editing and screening) such that thousands of individual edits can be...says he’s “known of Addgene basically since the beginning and [has] been using Addgene ever since” and that...