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TypeBlog Post... and memory. We’ll cover these in a future blog post, so stay tuned! References Alpár, Alán, et al...Addgene blog Read all of our viral vectors blog posts Find tips for getting started with viral vectors...
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INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...the Addgene blog Browse all of our CRISPR blog posts Check out other CRISPR methods for bacteria Resources... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...simple procedure is often taken for granted. In this post, we take a moment to review what exactly you are...one of the most popular options, so most of this post is based on reading results from a NanoDrop. The... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... including plants, mammals, and insects. In this post, we will compare two of the most widely used systems... time, or reagents and equipment. Hopefully this post has provided some insight on these differences in... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...cells, but mammalian cells can perform human-like post-translational modifications that bacterial and most...effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are not equally... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...libraries can be quite challenging to use! In this post, I’ll walk you through how to prep and transduce...perform your pooled library screen…but that’s a blog post for another day. Using pooled libraries can be ... -
Getting to Know Expansion Microscopy
TypeBlog Post...applications from across disciplines now available. In this post, we’ll review the inspiration behind ExM, how it...samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should start... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...their interaction. You can read our previous blog post for more on BiFC principles and experimental design...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors... -
Hot Plasmids - August 2020
TypeBlog Post...please sign up here. Here's what you'll find in this post: Yeast-based protein evolution Fluorescent tools...from Chertkova et al., 2020. In their BioRxiv post, the authors generated and described several improved... -
DNA Purification Without a Kit
TypeBlog Post...acid purification methods not mentioned in today’s post? Let us know in the comments! Additional resources...protocols and tips Browse our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post... blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts Resources... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus... blogger, Luke D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post... This post was contributed by guest blogger Erik L. Snapp. Stop using EGFP/GFP for fusion proteins! Despite...Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find the Best Fluorescent Protein for...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...This post was contributed by guest blogger Tessa Cressey. The highly pathogenic Ebola virus belongs to...PMC1182658. Resources on the Addgene Blog Read Other Posts on Viral Vectors Check out Our Scientist's Guide... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...Grant_Chat Additional Resources Read other Career Posts on the Addgene Blog! ... -
Plasmids 101: Protein tags
TypeBlog Post... list more common tags than are described in the post, but have been categorized to help you better assess...Healthcare Life Sciences. Addgene pages and blog posts of interest: Which Fluorescent Protein Should I... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...This post was contributed by Jim Woodgett. Public Library of Science (PLOS) created a stir earlier this...(The Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ... -
New Viral Vectors - Spring 2025
TypeBlog Post...viral vector services and resources! This blog post was written by Brook Pyhtila and Brian O'Neill. ... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post...resources on the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources...