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...having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and ... on your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel ... and 2 in our Technical Design of a Western Blot post to make sure you have the optimal gel for your experiment...consider (see our “technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing... Splice variants Check literature Posttranslational modifications Check literature Highly...

...time we have saved researchers. In a recent blog post, we looked at how long it takes to make one plasmid...

...ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our ...

...Additional Resources on the Addgene Blog Check Out Posts on Synthetic Biology Learn about the Addgene, Seeding...

...Visit to China Read Zafira Castano Corsino’s Recent Post on the International Mentorship Program Learn How...

... or detail. By the end of 2023, we had over 900 posts, which were read by researchers all over the world...mean all over (Figure 3). We continue to add new posts every week, and we’ve made our popular blog series...

...visualization methods. Stay tuned for a future blog post to learn more about these techniques!  Direct vs...effective and reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique...

...This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful...between loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different...markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in high concentrations...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...proximity to the region to be edited. In this blog post, we will cover three ways to circumvent this limitation...and Mary Gearing contributed to the update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler...

...relationships you develop will lead! Read our blog post about fostering communication in remote collaborations...Blog Check out Our Career Topic Page Read Other Posts in the Transferable Skills Guide Developing Transferable...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create...collaborative environment. Think of student groups, PostDoc associations and specific projects where you share...

...This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital...somatic cells for reprogramming purposes. In this post, I’ll describe an episomal vector system that increases...Mukherjee. Kusumika (Kushi) Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with...

...Out Our Networking for Scientists Blog Posts Read Our Career Posts Read Joanne's Management for Scientist's...frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice giving ...

...Last updated Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle...different types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less ...

...Another factor to consider is the age of your culture post thaw. In general, the higher the passage number ...produce the virus, titer the virus (read this blog post for tips on titering your lentiviral prep!) and ...

...Improve the Research Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids...

...January 28. (Article). Read More: Other CRISPR Blog Posts ...

... major ones in Addgene’s repository later in the post. Advantages of prime editing Less constrained by...treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration of...plasmids! Find plasmids from David Liu's lab! This post was originally written by Jennifer Tsang in October...

...more about DETECTR Browse all of the CRISPR blog posts Download our CRISPR cheat sheet Resources on Addgene.org...

...sequencing results Above (top right corner of this post) is an example of a seemingly clean DNA sequence...

...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College...parallel assays with other FPs.   We hope that this post helps you in getting to know the state-of-the-art...