We narrowed to 602 results for: sam

1. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...Remember to quantify the DNA concentration of all the samples by gel or by nanodrop to assemble the  fragments...

2. Summer SciComm Series: Cognitive Load

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   ... way I’ve come up is this: It takes me about the same amount of time to read a 100-page genre fiction ...

3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...other hand, using a constitutive promoter in the same background resulted in a 20-fold variation of gene...

4. Human Germline Editing Using CRISPR

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   ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....

6. CRISPRainbow and Genome Visualization

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   ...plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing...

7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was the shape...

8. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and successful...

9. Making CRISPR Plasmids Using Fragmid

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   ...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting...

10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...fission yeast, Schizosaccharomyces pombe, have the same function as their counterparts in S. cerevisiae....

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate...

12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with...

13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...not go to medical school was devastating. At the same time, she has an entirely new path to discover and...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...

15. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...detected in cells electroporated with RNPs using the same guide. Nanoblades also had lower off-target effects...

16. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical...

17. Overcoming the Challenges of Lentiviral Production

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    ...dishes. While this option is attractive because the same procedure for a single 10 cm2 dish can be easily...

18. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...both lentiviruses and gamma-retroviruses use the same gene products for packaging, however, the isoforms...

19. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    ...requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload your data...

20. Fluorescent Proteins 101: Introduction to FRET

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    ...contain the donor and acceptor fluorophore on the same protein and are useful when a process affects the...