We narrowed to 610 results for: sam

1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...characterized and the system allows you to add this same antibody to any protein to get really beautiful ...

2. Dos & Don'ts When Publishing a Scientific Manuscript

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   ... journals or as many journals as possible at the same time. Take your time picking the appropriate journal...

3. Plasmids 101: Yeast Vectors

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   ...modify proteins yet they still share many of the same technical advantages that come with working with...

4. Human Germline Editing Using CRISPR

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   ...deletion in MYBPC3 donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....

6. CRISPRainbow and Genome Visualization

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   ...plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA engineering for genome editing...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...other hand, using a constitutive promoter in the same background resulted in a 20-fold variation of gene...

8. Visualizing Protein Turnover In Situ

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   ... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...

9. Summer SciComm Series: Cognitive Load

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   ... way I’ve come up is this: It takes me about the same amount of time to read a 100-page genre fiction ...

10. Troubleshooting Your Plasmid Cloning Experiment

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    ...Remember to quantify the DNA concentration of all the samples by gel or by nanodrop to assemble the  fragments...

11. Celebrating 15 Years of Scientific Sharing

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    ...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...

12. Antibodies 101: Conjugation

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    ... antibody will have been verified to perform the same as its unconjugated version, but if you find yourself...

13. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was the shape...

14. Career Coaching for Scientists: Why and Where Do I Find One?

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    ...Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and successful...

15. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...not go to medical school was devastating. At the same time, she has an entirely new path to discover and...

16. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...requires lots of data. Get your community involved in sampling or bioinformatics analysis or upload your data...

17. Fluorescent Proteins 101: Introduction to FRET

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    ...contain the donor and acceptor fluorophore on the same protein and are useful when a process affects the...

18. Plasmids for Endogenous Gene Tagging in Human Cells

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    ... For an N-terminally tagged protein, we used the same strategy, inserting the tag and the linker preceding...

19. Overcoming the Challenges of Lentiviral Production

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    ...dishes. While this option is attractive because the same procedure for a single 10 cm2 dish can be easily...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...both lentiviruses and gamma-retroviruses use the same gene products for packaging, however, the isoforms...