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We narrowed to 1,010 results for: CAL;

Showing: 461 - 480 of 1010 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...asbestiform minerals such as erionite, are the only scientifically proven causes of the disease. There is no question... question on this point within the medical and scientific communities, and the link between asbestos and.... New techniques are currently being tested in clinical trials to detect mesothelioma earlier than ever...the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from injury, ...brought a lot of promise to a field that has historically been full of bad news and dreadful prognoses...Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources...

  2. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements. Many methods...be used for an array of applications including clinical diagnostics (e.g. pathogen or virus detection)...points out that the protocol is not approved for clinical use at this point, they hope that the protocol...this ability to create a DNA detection platform called DETECTR. How does DETECTR work? DETECTR works similarly...mixed population for an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...a reporter or tag that can then be detected.  Typically, the reporter is an enzyme such as horseradish...level of antigen present in the sample and is calculated by extrapolating from the standard curve. Because...include undesirable molecules that may bind non-specifically to assay components and increase background ...amount of antigen present in the sample and is calculated by extrapolating from the standard curve. Scientists...antibodies are used.  Competitive ELISAs Scientists typically use a competitive or inhibition ELISA when measuring...While the precise setup of the method can vary, typically users first coat the multiwell plate with a reference...

  4. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...DnaA binds multiple specific sites within the ori called DnaA boxes. These boxes come in both high- and ...Created with BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits...itself. RepA binds to directly repeated sequences called iterons located near the ori and directly interacts... the protein to be translated. An antisense RNA called Inc, also encoded by the ColIb-P9 plasmid, is complementary...between iterons on different plasmids in a process called "handcuffing," which blocks replication. ColIb-...Replication Browse other Plasmids 101 Posts Get practical molecular biology help with our Molecular Biology...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical barriers...’ve turned the available into the accessible. Physical access Addgene is located in the USA, but our ...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...Digest Analysis We also introduced guides — practical primers to help users get started with tools like...increasing the accessibility of our repository, from physical materials to useful information, but we’re not...

  6. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...defined chunks, called stories, and work on stories during short periods of time called sprints. The team...code, we might be calling something a sample and in other places, we might be calling it an aliquot. This...follow an agile methodology of software development called Scrum. The Scrum framework is based on an iterative...ourselves. We are also able to consider different technical approaches that may come up during discussion....

  7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...improved, by adding a nuclear localization sequence (SV40 Large T nuclear localization sequence) and expressing...asymmetric target site core but its 8 bp length that is critical for Cre and FLP function. Because recombination...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...this plasmid mix results in sparse labeling of cortical neurons and their synapses.   How can you...control   Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry...

  8. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...combining these two strategies allows you to specifically incorporate homo- or heterozygous mutations....in the field at the time but had never been systematically analyzed: introducing silent CRISPR/Cas9 blocking...DNA region? To this end we developed a technology called CORRECT, which, by undergoing two rounds of gene-editing...efficiently incorporated during HDR. We decided to systematically characterize this phenomenon and found a general...two successful mutations in a single cell. We calculated the likelihood of obtaining homo- and heterozygous...University. He currently works to understand pathological mechanisms of Alzheimer’s disease by developing...

  9. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...cryogenic electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target...limitations, the Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM...circles), magnetic bead (*), and spacers are labeled; scale bar: 100 nm. D) 3D model of the obtained structure...right). B) Protein samples protected from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of ...orientations, this could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated...evolved recombinases and prime editing. Nature Biomedical Engineering, 10.1038/s41551-024-01227-1. doi:...

  10. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...transformative biological discoveries and life-saving medical breakthroughs and undermine critical research ...Addgene as a trusted partner for archiving their biological materials during funding transitions. Enabling...private partners to create additional pathways for critical scientific work to continue.  Moving Forward ...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...-orbital AAV injection at 4 weeks. Cortical layers (L1-6) and scale bar are indicated. B) Specificity,... core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the cell surface by a GPI-anchor.... types in the brain can be a challenging — but critical — factor for successful experiments or therapies...enhancer-AAV vectors targeting specific classes of cortical and striatal interneurons (Furlanis, Dai, Leyva...PV-positive cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing BiPVe3...

  12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...one be interested in state machine technology? Biological research has produced a massive amount of information...processes that are influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM...prescribed region of DNA termed the “register”. Each chemical input to an RSM drives expression of a distinct...to record all permuted substrings of a set of chemicals. Specific examples of arrangements that should... record all permuted substrings of up to seven chemical inputs are given in Table S2 of our paper. The... He is broadly interested in biocomputing and biological circuits.     References 1. N. Roquet et al.,...

  13. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...Translocation Reporters (KTRs) whose cellular localization serves as a proxy measurement of kinase activity...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...which is further fused to a bipartite nuclear localization signal (bNLS) and a nuclear export signal (NES...phosphorylatable. Prior to phosphorylation, the KTR localizes to the nucleus, but following phosphorylation ...breakdown (without the nucleus, there’s no nuclear localization to monitor). Whether you’re looking for a ready-to-use...

  14. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ... first, which he called aequorin, gives off a faint blue light when it binds calcium ions, and the second...second, which we now call GFP, absorbs that blue light and glows green. In the late 1980s, another researcher...gene transcription, as well as to track protein localization. And as it turns out, he was right! He began.... He even obtained samples of certain ones from local pet shop aquariums in Moscow where he lived! His...

  15. Editor's Choice, September 2016

    Type
    Blog Post
    ...re tired of calculating dilutions or you can’t quite remember the hazards of that chemical in your obscure...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...can find the rest of our September posts in chronological order here: Four Things I Learned at the 10...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ...by the use of AAVs? 1) Combined functional and anatomical mapping of circuits In our lab, we use AAV-DREADD...these complexes are so robustly expressed that anatomical assessments can be easily performed too. While...identified this feature of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron...Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics vs. ...should strengthen its appeal for the scientific and medical communities. Many thanks to our guest blogger ... Floris G., et al. "A fourth generation of neuroanatomical tracing techniques: exploiting the offspring...

  17. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...human speech near the laminar airflow, the human scalp, sneezing, coughing, etc. Also, a constant in-flow...contamination Detection of mycoplasma by naked eyes or optical microscope can be very difficult, so how do you...cells detect mycoplasmas, they trigger a series of chemical reactions and this induces a noticeable color ...contamination, before using the cell lines for assay or analytical purposes. Use 0.1-micron filters as are they ...Fumigate the laminar airflow and the lab facility periodically. The CO2 incubators, used for maintaining cell...cleaned regularly and checked for contamination periodically. For more tips, check out Addgene's video Getting...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...depositors; Gizela, the lab member who knows how the physical kits are made and shipped; Chiara, the tech transfer...repository, performing quality control, and providing technical customer service. Team members are highly trained...problem? Test a hypothesis? Whether you officially call it a “project team” or not, most scientific collaborations...But this is a blog post, so here are just a few critical features based on my own experience: Leadership...should be on the team. As a team leader, I’ve (diplomatically) booted people off teams. I’ve also kicked ...like meetings, but run well, meetings can be a critical part of any successful team. For reasons I don...

  19. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...optimization is performed to take the drug to the pre-clinical and human trial phases.   So how does CRISPR factor...enables researchers to select and generate physiologically relevant models. In complex diseases, it can...standard is to test the drug on models which are genetically proficient and deficient for the target. CRISPR...discovery – it’s an exciting time to be in the biomedical research field! References and Resources References...

  20. Hot Plasmids - October 2022

    Type
    Blog Post
    ... developed an improved voltage indicator (GEVI) called JEDI-2P. Using a custom, high-throughput screening... the protein sequence of the predecessor GEVIs (called ASAP 1 and 2), they found an indicator that is ...approach and outcomes of the screen for improved genetically-encoded voltage indicators (GEVIs) based on the...optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts ...selective for K+ over other Na+ and other cations. B) Cortical slice of HcKCR1-EYFP and tdTomato expressed layer...
Showing: 461 - 480 of 1010 results