We narrowed to 470 results for: Lif

1. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

2. Sequencing Options for CRISPR Genotyping

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   Blog Post

   ...address these concerns as CRISPR use grows across the life sciences and to establish standardized validation...

3. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ... inserts can be made by PCR using primers that amplify the DNA sequence of interest and flank it with ...

4. Antibodies 101: Flow Cytometry

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   Blog Post

   ...antibodies can bind to a primary antibody thus amplifying the signal.       Intracellular protein targets...

5. Which Fluorescence Microscopy Technique is Best for Me?

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   ...or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...

6. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   ...proteins, as it enables tracking throughout the lifetime of the cell.     The past 15 years have seen a...

7. A Needle in a Base-Stack: Cas9 Structural Biology

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   Blog Post

   ...Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes...

8. CRISPR 101: Multiplex Expression of gRNAs

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   Blog Post

   ...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...

9. How to Deposit Your Plasmids with Addgene

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   Blog Post

   ... that the material I am submitting to Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I ...

10. CRISPR/Cas9 FAQs Answered!

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    Blog Post

    ...DNA Polymerase for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the...