...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...If you anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution ...

...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting...DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile...Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody and dispose of it...

...business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...

...back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even...

... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...

... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...

...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...

...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...

... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...

...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...

...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

...is important to know that the student presenting right before me had been assigned a structure paper with...

...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...

...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...

... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it...

...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown...

...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated...

...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever...

...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared...

...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target...

...with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing...

...bacteria deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number...

...some classic protocols on Protocol Online that you might find useful for your research. LabGuru Website: ...

...hosts. Also, self-transmissible plasmids may have high copy numbers and reduced metabolic load (Meyer 2009...

...amplification in the host cell. This enables relatively high and long-term expression of the reprogramming factors...