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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 990 results for: IGH@

Showing: 921 - 960 of 990 results

  5. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a question about a...

  14. Plasmid Cloning by PCR

    Type
    Blog Post
    ...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

  19. Lentivirus ddPCR Titration

    Type
    Protocol
    ...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown...

  25. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

  26. DNA Quantification

    Type
    Protocol
    ...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

  28. Antibodies 101: Isotypes

    Type
    Blog Post
    ... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

  33. Summer SciComm: Preprints

    Type
    Blog Post
    ...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

  35. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

  36. Working with Nuclear Receptors

    Type
    Blog Post
    ...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...
Showing: 921 - 960 of 990 results