We narrowed to 499 results for: Ank;

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   ...potential negative consequences of the tag. Many thanks to our guest blogger, Joachim Goedhart! Joachim...

2. The Fluorescent Vegetables in Aptamer Soup

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   ...PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification...

3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers Shravanti Surest from Iowa...

4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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   ...Cas-blocking mutations into the HDR repair template. Many thanks to our guest bloggers Dominik Paquet and Dylan ...

5. Antibodies 101: Introduction to Immunofluorescence

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   ...all claim to target your protein of interest. Thankfully, there are a number of websites compiling antibody...

6. Quick Guide to Near-Infrared Fluorescent Proteins

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   ...choosing the perfect one for your experiment!   Thank you to our guest blogger! Daria Shcherbakova is ...

7. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...measurement is often more accurate as a loading control. Thanks for joining us in this exploration of western blot...

8. Filming Science Videos in the Age of Social Distancing

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   ... but we needed someone else. Design: Jennifer thankfully volunteered, and we decided to focus on a protocol...

9. Multiple Plasmids at a Low Price: Kits from Addgene

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   ...peptides including antibiotic efflux pumps and ankyrin seven-repeat proteins: Sjuts, Hanno, et al. "Molecular...

10. Using Phosphoserine to Study Protein Phosphorylation

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    ...phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie Niemi! Natalie Niemi, ...

11. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...catalyze directional cloning of DNA parts that are flanked by orthogonal versions of the attB and attP sites...

12. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ...high-concentration, column-purified DNA template.   Many thanks to Channabasavaiah B. Gurumurthy, Masato Ohtsuka...

13. Technologies Enabled by NanoLuc® Luciferase

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    ...to see how you utilize them in your group! Many thanks to our guest blogger Kyle Hooper. A former Technical...

14. How to Design Your gRNA for CRISPR Genome Editing

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    ...experimental design, execution, and analysis. Many thanks to our guest blogger John Doench! John Doench is...

15. Plasmids 101: Dimers and Multimers

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    ...kb). Image generated by SnapGene software.  Thanks to increasing affordability and accessibility, ...

16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...submit your validation data after your experiment! Thanks to Addgenies Brian O’Neill and Ina Ersing for contributing...

17. Antibodies 101: The Basics of Western Blotting

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    ...system the sandwich is completely submerged in a tank filled with a transfer buffer formulated to conduct...

18. Generating Mouse Models Using CRISPR/Cas9

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    ...much to learn from biology in all its forms! Many thanks to our guest bloggers, Wenning Qin and Haoyi Wang...

19. Plasmids 101: Restriction Cloning

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    ...completed using a single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites...

20. Finding Your Perfect Job After University

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    ... to study a MSc, so I had to take out a personal bank loan. I saved money from my role in Industry and...