We narrowed to 712 results for: BASE
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TypeBlog Post...brainstorming strategies for using combinations of AAV-based molecular tools for targeted neuronal manipulation...
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Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post... it detaches from the gills and latches onto the base of the tongue where it pierces the tongue and drains... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post... Using myIDP, you can identify suitable careers based on your current interests and skillset. With this... -
Science as Art
TypeBlog Post...Daniel Stoupin is a PhD scientist and photographer based at the University of Queensland, whose outstanding... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...be continually improving the support we provide based on your valuable feedback. This is Addgene after... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...results could be biased for two reasons: 1) they are based on the US Occupational Employment Statistics (OES... -
SunTag and Fluorescent Imaging
TypeBlog Post...fluorescence, SunTag can also be used to improve CRISPR-based activation of target genes, but we’ll save that ... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...Addgene’s new approach of working with the industry-based scientific community. Conversations with children... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post... sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations in single... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post... control process for incoming plasmids can vary based on the contents of a plasmid, the first step is ...that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization of ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post...delving into new topics and expanding your knowledge base extensively, you open doors to fresh insights and... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...Biological Society, England The Biological Society based at the University of Oxford currently has around... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...vector size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...chromatin organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post... the number of plasmids requested by scientists based in Europe over the first five months of 2014 reaffirms... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...Listed - a CRISPR Screen Tool Green Listed is a web-based software tool freely accessible though http://greenlisted.cmm.ki.se...: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-... -
Important Considerations When Using AAVs
TypeBlog Post...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used...interested in using chemogenetic and optogenetic AAV-based tools to study neural control. Surprisingly, little... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry behind the...recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question, mapping and characterizing... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...Transposable elements are split into two classes based on their mechanism of transposition: Class I TEs...Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in... -
Protocol - Bacterial Transformation
TypeProtocol...more susceptible to either chemical or electrical based transformation, generating what are commonly referred...