Addgene

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Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

Type

Blog Post

... control process for incoming plasmids can vary based on the contents of a plasmid, the first step is ...that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization of ...
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

Type

Blog Post

...Addgene’s new approach of working with the industry-based scientific community. Conversations with children...
What's the Best Way to Elute and Store Your Plasmid DNA?

Type

Blog Post

... sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations in single...
Who Gives a Tweet? 9 Facts About Scientists on Twitter

Type

Blog Post

...results could be biased for two reasons: 1) they are based on the US Occupational Employment Statistics (OES...
SunTag and Fluorescent Imaging

Type

Blog Post

...fluorescence, SunTag can also be used to improve CRISPR-based activation of target genes, but we’ll save that ...
Embracing Serendipity: A Crucial Element in the PhD Journey

Type

Blog Post

...delving into new topics and expanding your knowledge base extensively, you open doors to fresh insights and...
A Better Way to Get Customer Support: The Help Center

Type

Blog Post

...be continually improving the support we provide based on your valuable feedback. This is Addgene after...
Networking and Inspiring Young Scientists at Freshers Fairs

Type

Blog Post

...Biological Society, England The Biological Society based at the University of Oxford currently has around...
pSiM24: Simplifying Plant Genetic Engineering

Type

Blog Post

...vector size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication...
Mapping the 4D nucleome with CRISPR/Cas9

Type

Blog Post

...chromatin organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging...
London Calling - Addgene’s New Europe Office in London

Type

Blog Post

... the number of plasmids requested by scientists based in Europe over the first five months of 2014 reaffirms...
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Type

Blog Post

...For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry behind the...recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question, mapping and characterizing...
Custom CRISPR Screens & the Green Listed Software

Type

Blog Post

...Listed - a CRISPR Screen Tool Green Listed is a web-based software tool freely accessible though http://greenlisted.cmm.ki.se...: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...
Important Considerations When Using AAVs

Type

Blog Post

...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used...interested in using chemogenetic and optogenetic AAV-based tools to study neural control. Surprisingly, little...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

...Transposable elements are split into two classes based on their mechanism of transposition: Class I TEs...Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in...
Protocol - Bacterial Transformation

Type

Protocol

...more susceptible to either chemical or electrical based transformation, generating what are commonly referred...
CRISPR Challenges: Standardization and Homology Directed Repair

Type

Blog Post

...repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without the need...
Oh, The Places You Can Go: Careers in Science Communication - Product Development

Type

Blog Post

... creating the outline of a college level course based on their research interests. “So for me, I researched...
Viral Vectors 101: Parts of the AAV Transfer Plasmid

Type

Blog Post

... an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The...
Plasmids 101: What is a plasmid?

Type

Blog Post

..., etc). The cloning method is ultimately chosen based on the plasmid you want to clone into. Regardless...

Showing: 461 - 480 of 706 results

Results per page:

Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

What's the Best Way to Elute and Store Your Plasmid DNA?

Who Gives a Tweet? 9 Facts About Scientists on Twitter

SunTag and Fluorescent Imaging

Embracing Serendipity: A Crucial Element in the PhD Journey

A Better Way to Get Customer Support: The Help Center

Networking and Inspiring Young Scientists at Freshers Fairs

pSiM24: Simplifying Plant Genetic Engineering

Mapping the 4D nucleome with CRISPR/Cas9

London Calling - Addgene’s New Europe Office in London

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Custom CRISPR Screens & the Green Listed Software

Important Considerations When Using AAVs

Plasmids 101: Using Transposons in the Lab

Protocol - Bacterial Transformation

CRISPR Challenges: Standardization and Homology Directed Repair

Oh, The Places You Can Go: Careers in Science Communication - Product Development

Viral Vectors 101: Parts of the AAV Transfer Plasmid

Plasmids 101: What is a plasmid?

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