We narrowed to 706 results for: ESC;
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TypeBlog Post...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...
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Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... Gal4/UAS system to knock down gene expression, rescue knock out mutations, and overexpress viral genes... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...and host our expression data (western blots or fluorescent microscopy) on the material pages associated ... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka... -
Resume Writing for Non-academic Science Careers
TypeBlog Post... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using... -
How Addgene Creates Content
TypeBlog Post...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes... -
Plasmids 101: Inducible Promoters
TypeBlog Post...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations... -
Viral Vectors 101: Types of viruses
TypeBlog Post...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...trillion connections (synapses.) In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...