Addgene

We narrowed to 705 results for: ESC;

Showing: 461 - 480 of 705 results

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Multiplexed Overexpression of Regulatory Factors (MORF) collection

Type

Blog Post

...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Type

Blog Post

... Gal4/UAS system to knock down gene expression, rescue knock out mutations, and overexpress viral genes...
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

Type

Blog Post

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...
Resume Writing for Non-academic Science Careers

Type

Blog Post

... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using...
Plasmids 101: Inducible Promoters

Type

Blog Post

...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...
Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

...editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. ...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

... the exact nature of your mutations. A great description of the plethora of available sequencing options...
Top Requested Lentivirus and AAV of 2016

Type

Blog Post

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...
Viral Vectors 101: Types of viruses

Type

Blog Post

...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...trillion connections (synapses.) In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...
Flow Cytometry Readouts: Yes, No, and Everything in Between

Type

Blog Post

...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...
How Addgene Creates Content

Type

Blog Post

...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins ...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...worries, I've summarized the three strategies described in this post in the graphic below - including ...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

Showing: 461 - 480 of 705 results

Results per page:

Multiplexed Overexpression of Regulatory Factors (MORF) collection

Plasmids 101: Gram Negative and Positive Bacteria

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

Resume Writing for Non-academic Science Careers

Plasmids 101: Inducible Promoters

Building and Validating Recombinant Antibodies: A Case Study

Antibodies 101: Buffers, Storage, and Conjugates

GPCRs: How Do They Work and How Do We Study Them?

Multiplex Genome Editing with CRISPR-Cpf1

Screening for Successful Genome Editing with Digital PCR

Top Requested Lentivirus and AAV of 2016

Viral Vectors 101: Types of viruses

Viral Vectors 101: The AAV Retrograde Serotype

Flow Cytometry Readouts: Yes, No, and Everything in Between

CasPEDIA: A Functional Classification of Cas Enzymes

How Addgene Creates Content

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR 101: Non-Homologous End Joining

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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