We narrowed to 703 results for: ESC;

... Gal4/UAS system to knock down gene expression, rescue knock out mutations, and overexpress viral genes...

...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample...

...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using...

...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...

... board. Eventually, you get lucky and manage to escape, but you’ve lost a lot of time. It doesn’t have...

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...

...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ...

...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...

...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

...and host our expression data (western blots or fluorescent microscopy) on the material pages associated ...

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...

... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using...

...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

... the exact nature of your mutations. A great description of the plethora of available sequencing options...

...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...