We narrowed to 637 results for: ESP

1. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...decide on the best way to structure roles and responsibilities as we grew.” Initially, he spent the majority...

2. Finding and Joining Your Dream Lab

   Type

   Blog Post

   ...for a lab Studies show that job satisfaction (especially for scientists and technical people) is linked...

3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   ...extinction coefficient (EC) and quantum yield (QY) respectively. The theoretical brightness is calculated by...

4. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...services. There was an overwhelmingly positive response to the option of Addgene providing plasmids in...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...

6. Viral Vectors 101: Viruses as Biological Tools

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   Blog Post

   ...which means they do not stimulate a strong immune response when introduced into an organism. This feature...

7. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   ...are still many open questions to be clarified, especially with regard to licensing models. Direct sharing...

8. Communicating Your Science With Help From ComSciCon

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   Blog Post

   ...graduate students to become ambassadors for their respective fields. To help attendees launch new organizations...

9. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...scientists with me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna...

10. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing is robust, with...

11. Plasmids 101: Inducible Promoters

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    Blog Post

    ...normally inactive and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and...

12. Hot Plasmids - May 2022

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    Blog Post

    ...fluorescent protein by a specific transcription response element (TRE) or kinase translocation reporters...

13. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    ...previously possible. This advancement could be especially exciting for scientists working to visualize ...

14. Antibodies 101: ChIP

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    Blog Post

    ...then processing with a homogenizer, but bone or especially fibrous tissues may need extra steps.  Fragmenting...

15. Hot Plasmids - February 2022

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    Blog Post

    ...less than half the size of either Cas9 or Cas12a. Despite its small size, CasMINI is comparable in efficacy...

16. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    ... by emitting a specific wavelength of light in response to excitation by a laser, which is then read by...

17. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ...Read the associated prompt carefully, plan your response, and let your inner wordsmith shine through. The...

18. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type

    Blog Post

    ...sequence just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't ...

19. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    ...together encode the N and C terminal segments respectively of a GFP dependent Cre. The two segments come...

20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ...they actually are. Throwing out plates that don’t respond as expected, use of representative images, measuring...