We narrowed to 672 results for: FIE

1. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid Cut vector...

2. Hot Plasmids - October 2022

   Type

   Blog Post

   ...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...

3. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

   Type

   Blog Post

   ... position/role, was both knowledgeable in their field and eager to engage with the work of others. Having...

4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   ...at 37 °C. These short DNA fragments can then be purified for subsequent library preparation and high-throughput...

5. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   ...Aligning sequences with Snapgene    Once we’ve identified the features within a plasmid, we next verify...

6. Plasmids 101: Gene, enhancer, and promoter trapping

   Type

   Blog Post

   ...world, approximately 50% of predicted genes have unidentified roles as well (Vanni, et al). Is it true that...

7. Plasmids 101: What is a plasmid?

   Type

   Blog Post

   ...innovative solutions necessary for advancing the field.   What is a plasmid? At their most basic level...

8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   ...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...

9. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

   Type

   Blog Post

   ...piece of advice for those looking to break into the field of science communications-- Be able to talk with...

10. Some Like it Hot: Thermostable GeoCas9

    Type

    Blog Post

    ... of GeoCas9 by the Doudna lab has opened up the field of thermophilic bacteria to CRISPR/Cas9 genome editing...

11. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    ...gmail.com. Who knows, your story might just get “legofied.” Many thanks to our guest blogger, Dalila Cunha...

12. Management for Scientists: Managing vs. Leading

    Type

    Blog Post

    ...bringing and effecting solutions to both known and unidentified issues. There are many good resources on the...

13. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    ...Dissolve in dH2O and sterile filter unless otherwise specified. The above table lists some antibiotics commonly...

14. Choosing a Good Mentor for Scientists

    Type

    Blog Post

    ... By contrast, as a postdoc, you should choose a field that you may want to work on in your future career...

15. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    ...career in science communication, an established field of inquiry with which I was not yet familiar. The...

16. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

    ..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...

17. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    ... proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection...

18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    ...quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer Agreement...

19. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    ...blue-shifting more than others. During this process, they identified mWasabi, a bright, monomeric green fluorescent...

20. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ...Reverse transcriptases are critical tools for many fields of biology and enable the conversion of RNA templates...