We narrowed to 877 results for: HA

...beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified...predictors because they are just that: rare. “Nature has many genes where there are not rare codons and many...

...this crisis has highlighted the importance of access to quality materials and data. Addgene has some exciting...

...Research. Drug development was something Stegmeier has been interested in for a long time. “At an early ...leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization efforts...

...product packaging) for free! It has to be from their company and it has to be packaging (no coated tissue...

...Throughout my internship, my interest in scicomm has grown and now it feels like an awesome bionerd hobby...Networking Tips Here And when asked what tips he has for better science communication for non-scientists...

...in Biochemistry with a background in virology who has worked with both animal and plant viruses. Now focusing...compounds with various antimicrobial activities (1). She has developed a fast endophyte screening system and plans...

...overlap extension PCR. Although each type of cloning has its advantages, most scientists still encounter several...science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning...

...Avengers This year Addgene, where my wife Lianna has made her career as Senior Director of Biology, is...pre-clinical development for First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy...

...sciences research. The rare disease research community has certainly noticed: This year, the BeHEARD (Helping...McSweeney (right), a graduate student at Duke who has recently moved to Columbia University with David ...

... (SB) transposon system is one such system which has been designed to perform gene transfer in vertebrates...defective transposase found in fish. This transposase has been discovered in the genomes of many different ...

...enables you to discuss a person's performance or what has been learned, it helps to plan effective next steps...about areas they need to improve in. If a manager has concerns about an employee and the employee doesn...

...share reagents so freely with the academic community has clearly made a huge impact on this field. Advances...talks, I oscillated between amazement at how much has been done with CRISPR technology in just 2 years ...

...an organization such as COMPASS. Although COMPASS has a special interest in ocean science, they have good...Program at the University of California, Santa Cruz, has been turning scientists into confident and connected...

...Mentee. A Mentor will be more effective if the Mentee has done the self-reflection necessary to identify areas... you have nothing to give, think again. Everyone has knowledge to impart. An undergraduate can mentor ...

...trend in funding and space in academic research labs has more students looking for other options. While graduate... skills for alternative careers. Science Careers has developed a web-based, career-planning platform called...

...learn about antibodies? Our new antibodies animation has all the information! Link in bio. #STEM #antibodies...

...measured using visible light scatter The optical system has both visible and fluorescent light sources. Visible...autofluorescence from your target cell type or that has arisen from fixation. Also include an isotype control...secondary antibody control in which the primary antibody has been omitted from the staining protocol. This control...gating during data analysis.  We hope that this blog has provided you with an overview of flow cytometry for...

...CRISPR/Cas editing toolbox for C. elegans Recent work has further improved the efficiency of genome editing... (Farboud et al., 2019). Work from the Mello lab has dramatically improved RNP editing with linear repair...elegans (Ebbing et al., 2017). Because C. elegans has an AT-rich genome, it can be difficult to find an...whereas import of CRISPR technology into C. elegans has been a transformative innovation for our community...

...increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed probe sets that...high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly screen buffer conditions for... 200 mM KCl at pH 8.5. This optimization method has been used internally at NEB to screen additional ...additional ligases, conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase....

..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...