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We narrowed to 856 results for: HA

Showing: 461 - 480 of 856 results

  1. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed probe sets that...high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly screen buffer conditions for... 200 mM KCl at pH 8.5. This optimization method has been used internally at NEB to screen additional ...additional ligases, conditions, and formulations and has led to the development of the new HiFi Taq DNA Ligase....

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ..., organelles or other cellular impediments. FRET has been used in many applications, including drug discovery...up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it...synthetases is required and thus far, this method has proven most effective only in a few cell types (E...FRET measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you...

  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...the opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well ...scientists who pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes...kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic constructs...stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs with various...

  4. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... improve its stability in vivo. However, Spinach has proven useful in the engineering of riboswitches....syndrome (Strack et al., 2013). Since then, Spinach2 has been used to investigate intracellular viral DNA ...better choice for imaging than the monomer. Broccoli has been developed into a split aptamer that can be used...

  6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ... and allows targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As ..., the search for smaller, compact genome editors has become a key focus in recent years. TnpB nucleases...nuclease domains (HNH and RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single...

  7. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... newest kid on the block in the sequencing world has been long-read sequencing. Whereas the individual...auto-alignment, plus additional automation in the backend, has enabled the QC to keep up with the pace of deposits...plasmid. By working with the Data team, the QC team has also improved efficiency in many of their methods...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires...Once the average copy number for a given plasmid has been reached, negative feedback circuits reduce replication...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

  13. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify...in human pluripotent stem cells, a cell type that has been difficult to modify in the past.   Figure...

  17. An Addgene Summer Internship

    Type
    Blog Post
    ... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

  18. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So how does...PCR products. Keep in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically...

  20. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...library screen; instead I thought they would focus on “Has this person been consistent throughout their career...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...
Showing: 461 - 480 of 856 results