We narrowed to 770 results for: LIS;

...new empty backbones By Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's...the payload sequence into the genome. The team established that they could reprogram the genomic target ...

...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...

...amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands using ... I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi:...

...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...

...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

...doi.org/10.1073/pnas.94.16.8640 PMID: 9238030 Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003). Transcription...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...

...the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...

...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput screening...

...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...

...Ac5 Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091...Ac5 Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139...proteinopathy, ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde...Parkinson's Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...

...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated...

...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...

...processing of our sequencing data.”  Recently, Seqera published a case study on how Addgene has used Nextflow ...

... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates...

... away. Is your favorite science comic not on our list? Let us know by sharing it with us in the comments...

...University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄...