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We narrowed to 768 results for: LIS;

Showing: 461 - 480 of 768 results
  1. Finding Your Perfect Job After University

    Type
    Blog Post
    ...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...
  2. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...
  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...amplified a 500-600 bp product. The primers I designed (listed above) worked well and I got clear bands using ... I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi:...
  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...
  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...
  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...doi.org/10.1073/pnas.94.16.8640 PMID: 9238030 Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003). Transcription...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...
  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput screening...
  8. Technical Design of a Western Blot

    Type
    Blog Post
    ...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...
  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Ac5 Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091...Ac5 Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139...proteinopathy, ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde...Parkinson's Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...
  10. Antibody Guide

    Type
    Collection
    ...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated...
  11. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...pC-GoldyTALEN Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative... domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation when...the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences (N153AA, C63AA) and showed...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...regulation of nearby gene expression. The vectors listed below have the EF1α promoter for efficient mammalian... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  12. Rett Syndrome

    Type
    Collection
    ...Mouse line with conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced ....806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold A140V...A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU 808delC Arg270Glufs...Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold BO_DI 806delG G269Afs...G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts...Fibroblasts Unpublished (Link opens in a new window) Wendy Gold For more information on generating iPSCs using...Link opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in ...
  13. Synthetic Biology - Overview

    Type
    Collection
    ...George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff...Maharbiz Thorsten Mascher Kathleen Matthews Anastasios Melis Aindrila Mukhopadhyay Richard Murray Erin O'Shea..., integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways... Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  14. Microbiology Resources

    Type
    Collection
    ...for specific genes or applications that are not listed here. Addgene Microbiology Resources Plasmid Kits...sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. ...West Nile virus (WNV) Zika Don’t see your field listed? Know of some great plasmid tools for the community...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  15. Arf GTPase Family

    Type
    Collection
    ...ARF GTPase Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each...available for each gene. Click the gene name for a full list of plasmids available. Please note that some genes...GAP and GEF Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  16. Plasmids for Stem Cell Research

    Type
    Collection
    ...expression of human L-Myc, Lin28, Sox2, Klf4, Oct3/4, Glis1, and EBNA1 from separate plasmids An Efficient Non-viral...from three separate plasmids A Facile Method to Establish Human Induced Pluripotent Stem Cells From Adult...expressing human Oct4, Klf4, Sox2, with either c-Myc or Glis1 Efficient generation of human iPSCs by a synthetic...differentiation of iPSCs into cortical and motor neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons... Research Plasmids This table contains a general list of plasmids that have been used in Stem Cell research... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
  17. Fluorescent Proteins: FRET

    Type
    Collection
    ...molecules, and more! Popular FRET Pairs This table lists some popular fluorescent proteins used as FRET pairs...with their properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor...designing your experiment, be sure to refer to published reports and include appropriate controls to help...Calibration Standards The articles or collections listed below feature plasmids with positive and negative...controls for many FRET pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live and Fixed Cells Additional...
Showing: 461 - 480 of 768 results