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We narrowed to 772 results for: LIS;

Showing: 461 - 480 of 772 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...doi.org/10.1073/pnas.94.16.8640 PMID: 9238030 Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003). Transcription...vectors, they offer the benefit of having well-established protocols for fast and efficient generation of...large-scale, high-titer preps. This is thanks to established retroviral production cell lines that are able...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in high-throughput screening...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...weight of the proteins you’re interested in. Table 1 lists the most common types of gels for SDS-PAGE running...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Ac5 Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091...Ac5 Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139...proteinopathy, ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde...Parkinson's Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde ...

  12. COVID-19 Resources

    Type
    Collection
    ...of spike protein mutations. Research Articles : A list of free access articles on COVID-19 research. Protocols...be made available as soon as possible. For a full list of searchable plasmids, see the table below. SARS-CoV...generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids...see our Plasmid Distribution to Industry page. The list of plasmids available to industry is continually... cytokine storm response in the body. For a full list of cytokines and related plasmids see our Immunology...SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing SHERLOCK...Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR...

  13. Antibody Guide

    Type
    Collection
    ...two most common types of ELISAs are direct or sandwich ELISAs. For direct ELISAs, an antigen or protein ...expression and assay efficacy. ELISA Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that...differentiate between proteins. HRP is also used in ELISAs, which only measure a single antibody signal. Here...relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein...Quantification methods, such as western blots or ELISAs, are used to quantify relative amounts of protein...standard curves to quantify exact amounts of proteins. ELISAs quantify a single protein per assay and typically...signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody and a primary conjugated...

  16. We’re Going Viral This Week!

    Type
    Blog Post
    ... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...pC-GoldyTALEN Golden Gate TALEN 2.0 36033 pTAL5-BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative... domain of a TALE, along with strong nuclear localisation tags and repress transcription initiation when...the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences (N153AA, C63AA) and showed...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...regulation of nearby gene expression. The vectors listed below have the EF1α promoter for efficient mammalian... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
Showing: 461 - 480 of 772 results