...proteins, as it enables tracking throughout the lifetime of the cell.     The past 15 years have seen a...

...or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...

... for the ability of the plasmid to be copied (amplified) by bacteria, which is an important characteristic...sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers can... using bacteria, which can uptake plasmids and amplify them during cell division. Because bacteria are... make proteins and other molecules essential for life. Scientists often “sequence DNA” to identify the...pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached to a glass slide... other. These attached DNA templates are then amplified again producing ~1,000 copies of each template...

...Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes...

... that the material I am submitting to Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I ...

...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...

...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

...discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then insert...restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested and ligated...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...). To do this your DNA fragment must first be amplified with specific Gateway attB1 and attB2 sites attached...

...replication-competent virus increases with each round of amplification. What is RCA? RCA stands for r eplication c ...crossover event increases with each round of amplification. Where can I find information about using the...

...Rep78, Rep68, Rep52, Rep40 - required for the AAV life cycle; VP1, VP2, VP3 - capsid proteins). When constructing...

...interest using PCR. This is accomplished by PCR amplifying the insert sequence using primers that contain...