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We narrowed to 882 results for: NSI

Showing: 461 - 480 of 882 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    ...you reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification...advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due... faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to purify DNA using a ...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...of looking for The One Method to Rule Them All, consider the following four questions to figure out which...than it is to design one demonstrating mitosis.   Consider also the amount and type of information you want...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...luck, and always keep going. Appendix DNAse Hypersensitive Sites and Intergenic CRISPRs Yours truly generated... In reality, cellular DNA is a complex three-dimensional globule of chromatin. Chromatin is a combination...bowtie2 -f -x HG19_GENOME --local -f -k 10 --very-sensitive-local -L 9 -N 1 -U GRNA_23MERS -S GRNA_HITS.sam...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...point mutation that increased the fluorescence intensity and photostability of GFP. This also shifted its... blog, you should! He's got great articles and insight on the newest tools and techniques in fluorescent...

  5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...important to note that in Morgens et al., guides were considered toxic if they were depleted from the screen. ...sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription factor... significant difference in depletion of ricin sensitizer genes identified with short and long guides. ...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently undergo...fluorescence for readout may render this approach less sensitive in most applications. Learn About Fluorescent ... sensor could save precious minutes in a time sensitive detection assay. In 1999, Grate and Wilson paired...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...When choosing beads, there are several factors to consider. Agarose beads are sponge-like and vary in shape...express the protein of interest, such as a sample transiently transfected or stably infected with the protein...appealing, but the disadvantages must be carefully considered before using. For example, the tag may affect...

  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... the virus and is less affected.  Altering sensitivity to serum. Different pseudotyped viruses are differentially... protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs) on a cell surface...CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti-, and spumaviruses to human...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...pTargetF system consists of one plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive replicon...changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...exception) necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids...are often many more genetic strategies) and then consider whether traditional cDNA overexpression is suited...eBook Resources on Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources...

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ... bins together at a mixer. We call this Quorum Sensing, after the phenomenon in bacteria that occurs when...gene expression in response to changes in cell density. At the mixer, they meet other Mentors and Singletons...briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks The last stage is Exponential Growth...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...?" is an open-ended list of proteins. Instead, consider "how do proteins X and Y regulate the Z pathway...that is easily visible from the back of the room. Consider going beyond the whiteboard. You may wish to create...blood sugar. Find an energy bar that you like and consider eating one before your chalk talk. Bring your ...

  15. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...of the desired T-A base pair. Figure 1: Transition mutation (C>T, G>A, A>G, and T>C) installation...also constructed smaller vectors suitable for transient assays using protoplast transformation. Cytosine...72 hours post-transformation/transfection in a transient assay. For stable transformation, regenerated ...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...clone. You will also need to use a E. coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene... the CcdB protein inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain ...sequences, start codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...place and give you some reasons why you should consider using Reddit to share your science. While Reddit...permanent reference by the Winnower. The only small downside to the AMA from my end as an organizer/promoter... general advice on how to meet people, how to transition out of academia, and other science career topics...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...retroviral backbones. On some occasions, shRNAs are transiently expressed using either AAV backbones or species-specific...synthetic, mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro...time you need to knock down a gene, maybe you’ll consider using RNAi! References and Resources References...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...material, which ensures the editor will only be transiently present in transduced cells, limiting off-target.... (2024). Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes...CRISPRoff system — a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that...
Showing: 461 - 480 of 882 results