We narrowed to 609 results for: SHE
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TypeBlog Post...Bulina, M. E., Chudakov, D. M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V.,...
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Visualizing Translation at the Single Molecule Level
TypeBlog Post...causing it to appear yellow. The act of translation pushes NLS-PCP-GFP off of the mRNA, leaving only a red... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...experiment, the DNA sample being sequenced must be sheared into smaller fragments that are then barcoded for... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...properties. Find these voltage biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;... -
Finding and Joining Your Dream Lab
TypeBlog Post...collaborations. Alternatively, perhaps the lab publishes and presents regularly with labs around the world... -
Ligation Independent Cloning
TypeProtocol...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ... -
Centrifugation
TypeProtocol...centrifuge and investigate the issue. When you are finished spinning your samples, carefully remove them from... -
How Addgene Creates Content
TypeBlog Post... the example of our Antibody Guide, which was published alongside our new antibody services in the spring... -
Hot Plasmids - February 2022
TypeBlog Post...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... the buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed ... -
Antibodies 101: Isotypes
TypeBlog Post...Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes that each have... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 blog posts Resources... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... from this collection in your experiments and published your data, please fill out this form and we’ll... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...of endogenous signaling activity in cells. Let’s shed some light on the key components of OptoNBs, how... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...: Zoomed-in view of traces corresponding to the dashed box above. The number of action potential (AP) ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...