We narrowed to 537 results for: SHI

1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...the Hirsh family, we are able to take greater ownership of this process with in-house sequencing! In addition...

2. Hot Plasmids and Viral Preps - January 2021

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   ...AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical...

3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ...Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow or orange-red...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

5. Hot Plasmids - October 2020

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   Blog Post

   ...available as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...

6. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   .... PubMed Central PMCID: PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based ...

7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...

8. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   .... Since makerspaces are generally funded on a membership model - everyone contributes each month - with...

9. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...attention of the audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good...

10. Optogenetics Guide

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    Guide

    ...PsChR1 Red-shifted chloride-conducting channel from Proteomonas sulcata 540 Phobos Blue-shifted iC++ variant...467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws Red-shifted, light-driven...kinetics: Examples: ChETA, C1V1(t/t), ChrimsonR Red-shifted peak action spectra Examples: VChR1, C1V1(t/t),...amplitude Examples: eNpHR, eNpHR2.0, eNpHR3.0 Red-shifted peak action spectra Example: Jaws Browse Halorhodopsin...K176R mutation. 590 PsChR2 High-efficiency blue-shifted channelrhodopsin from Platymonas subcordiformis...2014. Noninvasive optical inhibition with a red-shifted microbial rhodopsin. Nat Neurosci. 17(8):1123-9... Yizhar O, Hegemann P, Deisseroth K. 2008. Red-shifted optogenetic excitation: a tool for fast neural ...

11. Common Injection Routes in Mice

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    Blog Post

    ...131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. ...

12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

13. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...emerged as a particularly useful tool for those wishing to run larger panels: five laser spectral flow ...

14. 10 Basic tips for mammalian cell culture

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    Blog Post

    ... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)...

15. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based on Fuguo ...

16. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...grow the bacterial strain in which the plasmid is shipped. Copy number (if known), antibiotic resistance,...

17. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... our understanding of the structure-function relationship of the central nervous system under normal and...

18. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...against the database. Another benefit is that a frameshift mutation present in the ORF is readily apparent...

19. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...

20. Getting to Know Expansion Microscopy

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    Blog Post

    ...they want to understand the ultrastructural relationships that influence everything from cell division...