We narrowed to 542 results for: SHI

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   ...Sathyan V, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH,  Begeman A, Stark JC, Shon DJ, Fox DM, Puschnik...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...the Hirsh family, we are able to take greater ownership of this process with in-house sequencing! In addition...

4. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ...Goltstein PM, Portugues R, Griesbeck O. Putting a finishing touch on GECIs. Frontiers in molecular neuroscience...

5. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...

6. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   ...not currently provided. Eye protection or face shields are required whenever there’s a splash or spray...

7. A Look at Addgene's QC Process

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   Blog Post

   ...the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide indels or mismatches...

8. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   ...AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical...

9. Hot Plasmids - October 2020

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   Blog Post

   ...available as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...

10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...

11. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    .... Since makerspaces are generally funded on a membership model - everyone contributes each month - with...

12. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post

    ...attention of the audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good...

13. 10 Basic tips for mammalian cell culture

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    Blog Post

    ... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)...

14. Common Injection Routes in Mice

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    Blog Post

    ...131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. ...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

16. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...emerged as a particularly useful tool for those wishing to run larger panels: five laser spectral flow ...

17. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    ...potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based on Fuguo ...

18. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ...grow the bacterial strain in which the plasmid is shipped. Copy number (if known), antibiotic resistance,...

19. Light Sheet Fluorescence Microscopy

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    Blog Post

    ... our understanding of the structure-function relationship of the central nervous system under normal and...

20. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...online protocol repository hosted by the Nature Publishing Group. Like the previously mentioned sites, NPE...