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Showing: 461 - 480 of 612 results

  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... Scientists introduce an antibiotic resistance cassette within the coding region of the gene they are ...is best practice not to use the same resistance cassette for the mutation and for plasmid selection. Additionally... vector typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates...completely removes (in the case of Gateway cloning) one cassette. Counterselection allows the scientist to select...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ... background. Their new method uses a selection cassette containing genes for hygromycin resistance and...heat-shock inducible Cre to generate a self-excising cassette (SEC). To make it possible fluorescently label...into the genome. Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with...Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD...

  3. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...strewn across the floor. In the final wake it all settles down and the value of each item changes from what...individual; the  community as a whole has yet to settle on any true kind of value. I’ve dubbed this the...software design that is applicable to only one, or a subset of organisms. In only a few cases the software ... agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism agnostic”, “model... you can rule out any tool that limits you to a subset of specific genomes. These are most of the tools...

  5. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...targeting the genes responsible for both early onset and late onset AD. In this study, the group showed that...connected to the early onset of Alzheimer’s disease, as well as those that play into late onset AD (5).     ...

  6. Science Guides

    Type
    Guide
    ...genome, while others are restricted to certain gene sets. Read More Viral Vectors Find educational guides...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ... while pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays...). By cloning in two tandem copies of the gRNA cassette, one can reliably knockdown expression of multiple...such a purpose (Selle et al., 2020).  Targeting a subset of bacteria in a population Work from the Lu lab...found early success using CRISPR-Cas to target subsets of bacteria in population based on the presence...

  8. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... of a stop cassette upstream of the target gene in the targeting vector. The stop cassette is floxed, ...conditional systems Conditional knockouts or knockins A subset of transgenic mice, conditional knockout/knockin...so Cre-mediated recombination deletes the stop cassette, which permits expression of the target gene. ...

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...unbiased data. Therefore, we chose to examine a dataset of Liu lab plasmid requests from Addgene, as a ... requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for...and 03/11/20 -- 05/19/20 (the last date in our dataset) for the U.S. and Europe. To improve the stability...China. An important additional caveat is that our dataset contains plasmid orders from just one lab, which...

  10. Molecular Biology Reference

    Type
    Guide
    ...replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term either ... in many species and can be useful for a diverse set of applications - Plasmids can drive gene expression...Genetic Code The genetic code can be defined as a set of rules for translating the information encoded ...

  11. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

  12. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...gain fresh perspectives. Such activities foster a mindset that embraces the unexpected and encourages exploration...Serendipity is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative...serendipity into your research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...re-administration but I do not think the field has settled on ‘best practices’ for model organisms.    Q11...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses... own promoter   Kabadi et al Csy4-Cleavable Cassettes transfection gRNAs expressed from a polycistronic...co-transfection of Cas9 Nissim et alTsai et al PTG Cassettes transfection gRNAs expressed from a polycistronic...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    .... In the specific example of episodic memory, a subset of hippocampal neurons projects axons directly ...tracing has the added benefit of only labelling the subset of infected cells that also express Cre. Another...AAV vectors. Conclusion An ideal comprehensive toolset for neuronal tracing and network identification...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res...

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...and deposit individual documents or more complex datasets into the desired repository. They also support...experiment documented in RSpace using the RSpace datasets deposited in Figshare or Dataverse has access ...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential after development... gene was disrupted and silenced by a Lox-Stop cassette, resulting in the neurological deficits observed...Restoring the MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted ...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of tRNAs that...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...courtesy of IPI. IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies... that bound an amino acid region displayed by a subset of the RGD-binding integrins containing the αV ...
Showing: 461 - 480 of 612 results