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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: Sis;

Showing: 461 - 480 of 738 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... transmitted through the integrin, where it is resisted by the ligand embedded in the extracellular environment...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...lifetime imaging. B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ..., Y., & Peng, C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers...Ørom, U. a. V. (2023). Recent progress in miRNA biogenesis and decay. RNA Biology, 21(1), 36–43. https:/...

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...modification of interest, ordering them from a gene synthesis company such as IDT or Genscript, and cloning ...using adeno-associated virus (AAV)." Chromosomal Mutagenesis. Humana Press, New York, NY, 2015. 75-103. PubMed...

  6. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... steps: 1. Define your timeline Start with your thesis in mind. Imagine the kind of data your dream discovery...consultant and coach in Boston, MA. He specializes in assisting research scientists create productive and manageable...

  7. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...advantage of CRISPR epigenetic tools is their persistence and inheritance. CRISPR activators and repressors... culture. However, DNMT3A-induced methylation persisted throughout a 100 day experimental period, as this...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does... Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...protein tags. The basics These tags most often consist of an enzyme that has been mutated to block its...Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach to...you can separately detect these markers in your analysis. Lastly, there are fixable dye stains that react...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the complexity of the screen. For example, the analysis becomes easier, the number of cells needed is ...add value if you are addressing a well-defined, hypothesis driven research question. Examples of custom ...

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital. Biochemists...Fessenden! James D. Fessenden is currently an Assistant Professor in the Anesthesia Research department...
Showing: 461 - 480 of 738 results