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We narrowed to 731 results for: Sis;

Showing: 461 - 480 of 731 results

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency, Tropism and Axonal...Hosokawa, Y., & Konishi, H. (2014). A comparative analysis of constitutive promoters located in adeno-associated...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...generate more signal from low abundance targets. Analysis/Presentation Once you’ve captured your images,...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...be used for numerous applications, including transgenesis, gene-trap screens, and gene editing. How does... Verma, “Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...protein tags. The basics These tags most often consist of an enzyme that has been mutated to block its...Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical Biology, 3(6), 373–382. https://...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach to...you can separately detect these markers in your analysis. Lastly, there are fixable dye stains that react...

  11. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the complexity of the screen. For example, the analysis becomes easier, the number of cells needed is ...add value if you are addressing a well-defined, hypothesis driven research question. Examples of custom ...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital. Biochemists...Fessenden! James D. Fessenden is currently an Assistant Professor in the Anesthesia Research department...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ... carry one or more antibiotic resistance genes, which confer resistance to a specific antibiotic to the...to be resistant to the antibiotic. On the second plate, streak out a strain that’s not resistant to the... be resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the...Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant strain grows, it’s likely ... carrying them. The presence of an antibiotic resistance gene on a plasmid allows researchers to easily...for the selection of only those bacteria with resistance to that antibiotic - usually conferred by a plasmid... plasmid carrying the antibiotic resistance gene. The following protocol will allow you to make your own...

  15. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also like... Restriction...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation... Agarose Gel DNA Ligation Introduction Gel electrophoresis is the standard lab procedure for separating...pairs) for visualization and purification. Electrophoresis uses an electrical field to move the negatively...protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well combs Voltage ...solidified, place the agarose gel into the gel box (electrophoresis unit). Fill gel box with 1xTAE (or TBE) until... short DNA fragments is polyacrylamide gel electrophoresis (PAGE), which is typically used to separate...

  16. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...became automated with the discovery of a heat resistant DNA polymerase from the thermophilic bacterium...several cycles of denaturation, annealing, and synthesis. After the PCR is complete, the product can be...be verified based on size by gel electrophoresis . Protocol Video Watch the protocol video below to learn...temperature step-wise by 1-2°C. The rate of DNA synthesis is ~1-2 kb/min. The extension time can be adjusted...of DNA that we are interested in amplifying for analysis and manipulation. It is essential that the template...cations can also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the ...that are starting points for DNA elongation and synthesis. Taq DNA polymerase: Special DNA polymerase that...

  17. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...your restriction digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot...manufacturer's instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction...sequence (and where they will cut), use a sequence analysis program such as Addgene's Sequence Analyzer . ...visualize the results of your digest, conduct gel electrophoresis . Tips and FAQ Restriction enzymes MUST be ...Dam or Dcm methylase positive strains will be resistant to cleavage at certain restriction sites. See ...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....

  18. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...separated by denaturing polyacrylamide gel electrophoresis alongside serial dilutions of a standard antibody...Run SDS-PAGE and stain gel Day 1 or later: Image analysis Video Watch this instructional video to learn ...pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking platform Fume ...the bottom of the gel. Place the gel in the electrophoresis chamber and secure it. Note: Check the manufacturer...make quantifying the gel easier. Cover the electrophoresis chamber and attach to a power supply. Run the.... Turn off the power supply and unplug the electrophoresis chamber. Remove the gel from the chamber. Use...Addgene QC and would not be used. Section 3: Image Analysis Use ImageJ (Link opens in a new window) or a similar...

  19. Western Blot

    Type
    Protocol
    ...imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay...in appropriate volume of cold lysis buffer. Pro-Tips The volume of lysis buffer will vary depending on...will generally be between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location...the bands. Tips and Troubleshooting The optimal lysis buffer will vary depending on the sample type and...protein of interest. You may need to try a variety of lysis buffers to find the best for your target. The optimal...

  20. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis Introduction Restriction enzymes are naturally...and analyze the resulting fragments by gel electrophoresis . The pattern of the fragments on the gel can...enzyme or enzymes that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer ,...to analyze a restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid...manufacturer's instructions) Gel loading dye Electrophoresis buffer Verifying Total Plasmid Size -OR- Insert... tricks see the Restriction Digest and Gel Electrophoresis pages....
Showing: 461 - 480 of 731 results