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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 461 - 480 of 830 results

  1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

  3. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

  6. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  12. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

  14. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI is more ...“how much better it is once it gets fully up to speed.” He also comments that it is also surprising “ ...

  15. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...a lab that fits both work style and development needs. These two factors will provide a solid foundation...prefer a more competitive style to spur you to succeed or you might enjoy working closely with others ...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at the end...In order to produce gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains...stably express gag-pol and/or env , eliminating the need to deliver these genes on separate plasmids ( in...with contact to mucous membranes or broken skin. Needle sticks and ripped gloves are common points of entry...clinical-grade gamma-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing. Molecular...specificity of infection for CD4-expressing cells. Proceedings of the National Academy of Sciences , 94 (16)...the beginning of R, has a 5' cap structure, and proceeds through U5 and the rest of the provirus. Some ...

  17. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...most requested plasmids into lentiviral preps to speed up COVID-19 research even further. The following...different patterns of expression, researchers often need to determine these transduction patterns empirically...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...

  20. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...
Showing: 461 - 480 of 830 results