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1. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...

2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   Blog Post

   ...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ...

3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   Blog Post

   ...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...

4. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   Blog Post

   ...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...

5. With Our New Viral Service, We're Taking CRISPR Further!

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   ...and host our expression data (western blots or fluorescent microscopy) on the material pages associated ...

6. Four Factors that Differentiate the Stem Cell Field

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   ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...

7. Resume Writing for Non-academic Science Careers

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   Blog Post

   ... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using...

8. Plasmids 101: Inducible Promoters

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   Blog Post

   ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...

9. Multiplex Genome Editing with CRISPR-Cpf1

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   ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

10. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ... the exact nature of your mutations. A great description of the plethora of available sequencing options...

11. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

12. Building and Validating Recombinant Antibodies: A Case Study

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    ... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

13. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

14. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

15. Viral Vectors 101: Types of viruses

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    ...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...

16. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    ...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

17. Viral Vectors 101: The AAV Retrograde Serotype

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    ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

18. How Addgene Creates Content

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    ...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

19. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...worries, I've summarized the three strategies described in this post in the graphic below - including ...