Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 871 results for: lic

Showing: 461 - 480 of 871 results

  1. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer...

  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So let’s break ...transmembrane domain receptors, due to the seven alpha helices that transverse the cellular membrane. The receptor...engineered receptors to interact with small molecules to elicit a response in a certain cell type. Chemogenetics...

  5. How Addgene Creates Content

    Type
    Blog Post
    ...antibody and content team, we identified what applications should be included and what basic information...through an editing process and final check before publication.   Do research! Nearly everything we write entails...make figures in BioRender or use appropriately licensed and cited figures from papers or image sharing...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...sequences. Fusion PCR was performed to create an amplicon that  was cloned into a plasmid backbone for expression...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the key components of EvolvR and its potential applications. Challenges of existing mutagenesis methods...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...more than one mutation near the target site. Applications of EvolvR The Dueber Lab used EvolvR to discover...

  8. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...their reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  9. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...demonstrated that pathways projecting to the inferior colliculus (IC) are differentially transduced when using...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...as they do science outreach and engage with the public, and learn from the process of writing papers and...broader impacts for their work in education and policy and to draw attention and support to work in their...before you complete your work. If you’re giving a public talk, ask a friend who works in a different field...

  14. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...neuroscience method that lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded... expression in cultured neurons and mouse brain slices collected following intracranial injection of an... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    ...of DNA that can potentially complicate results of a downstream application such as quantitative PCR to...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA...

  16. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...methionine. The labeled proteins can be detected using click-chemistry that covalently links the azido containing... observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...

  17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement...self-process pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-... that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID can also be used...

  19. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...and in the intersection between science and public policy. Follow David on Twitter @driglar.     References...professor of economics at the Andrew Young School of Policy Studies at Georgia State University, is an expert...

  20. Working with Nuclear Receptors

    Type
    Blog Post
    ...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ...a broad range of reproductive, developmental, metabolic, and immune response programs. NRs also encompass...gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators Coregulators constitute...
Showing: 461 - 480 of 871 results