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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 869 results for: lic

Showing: 461 - 480 of 869 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... Specifically, viruses are able to infect and replicate in host cells, but they are susceptible to cells... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site.   The...

  2. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ... writing, lab budgeting, equipment acquisition, public speaking and personnel management?  If you are ...ask a lot of questions. Personality and style conflicts are a common reason for scientists to change labs...websites listing alumi and it is easy to use publication records, university websites, ResearchGate and...

  3. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ... be used as a fusion tag for a wide range of applications from traditional imaging to FRET and stochastic...fluorescent protein: structural characterization and applications in fluorescence imaging." Biochemical Journal...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...protein-only transduction method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only...Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP using Cre-lox recombination...diphtheria toxin, indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness...

  7. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ... mouse and rat lines. The Fenno Lab maintains a public folder (Link opens in a new window) of manuscripts...

  9. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... Glucose Glucose, methanol, glycerol Heterothallic mating Efficient Efficient Inefficient... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

  11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD Candidate...nephropathy, one of the major pathophysiological complications of diabetes mellitus. Recombinant human proteins...Biology of the NationalAcademy of Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior...

  12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...improve our shipment processes. After reading a publication describing how short term storage on dry ice ...closed dry ice storage container for 48 hours (to replicate how our viruses are shipped), where they would...exposed to CO2/dry ice. All samples were tested in duplicate. What we found Not all tubes (or rather, not ...

  13. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played.... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced... cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  15. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer...

  16. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...altering its function). HDR is crucial for certain applications, for example, correcting the allele that causes...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

  17. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...demonstrated that pathways projecting to the inferior colliculus (IC) are differentially transduced when using...not yet understood, one thing is clear: this application opens a lot of doors in neuroscience. The retrograde...retrograde serotype also has promising therapeutic applications; it can enhance therapeutic delivery across ...

  19. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...sequences. Fusion PCR was performed to create an amplicon that  was cloned into a plasmid backbone for expression...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  20. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this... Antibodies: Distinguishing Characteristics, Applications, and Information Resources. ILAR Journal 46:... the Addgene blog Learn more about research applications for antibodies in Intro to Antibodies Read about...
Showing: 461 - 480 of 869 results