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...yourself, and to address all of the other parts of your life, which, inconveniently, do not take time off when...

... inserts can be made by PCR using primers that amplify the DNA sequence of interest and flank it with ...

...Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen. Plant Physiol 178:989–1001...

... goal is to engineer the most miniscule parts of life, like proteins, to study or alter their function...

...address these concerns as CRISPR use grows across the life sciences and to establish standardized validation...

... transcribed by the RNA polymerase. Figure 1: Simplified promoter region during transcription. Created...CaMV35S Constitutive Plant Strong promoter from Cauliflower Mosaic Virus Ubi Constitutive Plant High-expression...

...or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human...

...proteins, as it enables tracking throughout the lifetime of the cell.     The past 15 years have seen a...

...antibodies can bind to a primary antibody thus amplifying the signal.       Intracellular protein targets...

...using BsaI, and promoter-gRNA fragments are PCR amplified for cloning into one of three Zeamays codon-optimized...

... that the material I am submitting to Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I ...

...Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes...

...genomes into hosts in order to continue their lifecycle and replicate. This feature can be harnessed for...Gamma-retroviral Packaging Systems The Retroviral Lifecycle Types of Viruses Viral Vector Elements More Viral...

...also resulted in a reduction of viral vector amplification and overall lower viral titers. Because of this...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...

...DNA Polymerase for my genomic PCR, but couldn't amplify the EMX1 gene using same primer you used in the...