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We narrowed to 760 results for: mpo

Showing: 461 - 480 of 760 results

  1. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...will link biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow...isotropic expansion depends on how well the gel components penetrate the sample, how well the gel polymerizes...

  2. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...interaction with other proteins.   It is important to note that research activities in a CURE must...opportunity to make new discoveries. Provost notes the importance of acknowledging student apprehensions about ...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experiments didn’t provide any information on other important factors such as what is the best promoter to use...successfully replicate published works. Of particular importance to these efforts is the open sharing of negative...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... step, you’ll want to ensure that your buffer components are compatible with your conjugate and conjugation...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...

  11. MXS Chaining

    Type
    Blog Post
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

  13. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  14. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...the type II Cas9-based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... care, but they need to know that you have an important life too. They will respect you if you show respect...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...

  17. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...toxin-antitoxin roles. Restriction modification systems are composed of restriction endonucleases and methyltransferases...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...
Showing: 461 - 480 of 760 results