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  1. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein...In both cases, the lipid membrane is the only component that is not dependent on viral genes. It is derived...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...will link biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow...isotropic expansion depends on how well the gel components penetrate the sample, how well the gel polymerizes...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...interaction with other proteins.   It is important to note that research activities in a CURE must...opportunity to make new discoveries. Provost notes the importance of acknowledging student apprehensions about ...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experiments didn’t provide any information on other important factors such as what is the best promoter to use...successfully replicate published works. Of particular importance to these efforts is the open sharing of negative...

  7. MXS Chaining

    Type
    Blog Post
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...the type II Cas9-based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...

  11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...solvent. This tightly-packed structure explains the importance of the entire GFP protein, which is almost completely...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... care, but they need to know that you have an important life too. They will respect you if you show respect...You can handle that, right? Big tip: this is an important topic to discuss and agree on before you get enmeshed...

  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... in a particular organism. Why is codon usage important? Translation and protein synthesis relies on transfer...(Mauro and Chappell 2014).  Ultimately, it is important to take a close look at your experiment and gene...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... passive mode, the stronger is the depressive component of the animal behavior. With the availability ...of viral vectors to fine tune gene expression temporally and spatially, exciting times are ahead for behavioral...

  16. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... and fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal...fluorescent proteins (smFPs). smFPs are literally composed of FPs fused to multiple epitope tags. Indeed,...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...implemented, impacted the entire company. It is important to note that the person who submitted the idea...making something the best it can be that it seems impossible to start at all. This is why perfectionists tend...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...virus’s most abundant proteins and a critical component for viral genome packaging. Addgene recommends...including in human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous...

  19. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...select for your vector. Some antibiotics block important cellular pathways shared by bacteria and eukaryotes...Finally, in some cases a researcher may choose not impose selection on cells and instead to use a vector ...

  20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...
Showing: 461 - 480 of 750 results