...of the University of British Columbia. When transitioning from an academic science career path to a non-academic... lectures? These transferable skills could be meaningful for your potential employer. For example, my ... Developing Transferable Skills During Science Training and So You Think You have Skills.   Get Science...graduate students in job searching. Start your career planning early, start now Don't wait until the day you ...

...Shortly after the initial article describing the cloning of firefly luciferase was published in 1985, several...Luciferase reporter assay The typical luciferase-containing plasmid is most often used to investigate the...cloned into a plasmid downstream of luciferase containing a stop codon, which is then transfected into ...into the desired cells. A hybrid mRNA transcript containing luciferase and the gene of interest permits translation...

... translation (Noren et al., 1989). In the late nineties, this was developed into an E. coli system, with...will be needed to introduce the gene of interest containing the chosen codon that will result in the incorporation... parameters the same for all three components, meaning that the final protocol may involve a sensitive... located at Oregon State University focused on turning GCE tools developed in the literature into robust...

...introduce frameshift mutations in genes of interest, turning expression off permanently. Viral vectors can ... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...Gabuzda D, Crowe SM, Mills J, Lewin SR, Brew BJ, Cunningham AL, Churchill MJ. Pathogenicity and immunogenicity...

...Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use...plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some of the most requested... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

...are available for request? Currently, plasmids containing all SARS-CoV-2 protein coding genes in E. coli...Addgene. A set of mammalian expression vectors containing various spike protein variants are also available...received Ginkgo’s first deposited set of plasmids containing SARS-CoV-2 protein coding genes in E. coli and...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

...drastically affects their stable folding and efficient functioning. But what about placing the mesophilic hosts ...consider them worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...coli! Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a...conventional academic settings, these are an eye-opening experience for cultivating a nuanced understanding...

...interest. Isotype controls are used to make sure the staining you see is due to target-specific antibody binding...binding rather than nonspecific background staining. Isotype controls are used in antibody-based applications...switch to expressing a different isotype, the intervening DNA sequence is deleted, which places the variable...domain gene cluster. DNA recombination can delete intervening heavy-chain constant domain genes, placing the...

...time-lapse images, quantitatively evaluating antibody staining, or counting cells are now routine, open-source...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...via globally established makerspace networks and training users via workshops, seminars, or hackathons greatly...research. The EU, for example, is calling for the opening of sources within the next few years, with the ...

...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...antibodies will exhibit off-target binding to unintended proteins. This is because monoclonal antibodies...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...

...posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find our molecular... this scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage and plasmid...techniques in basic molecular biology, plasmid cloning, and viruses. References Baltrus, David A., and...

...Addgene The links below pull up lists of plasmids containing the indicated gene, and you can subscribe to ...pages to learn when Addgene receives new plasmids containing these genes. As with the PIs above, many of these...your work. The links below direct you to pages containing lists of plasmids from the indicated publications...following articles are just a small cross-section spanning 20 years of research. DNA Cross-Bridging Shapes...

...involves two basic components: the pigments (anthocyanins), and the plant circadian clock. Petunias have...the biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work...comprehensive understanding of the genetic pieces underpinning these mechanisms – our project wouldn’t be possible...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

..., David Liu’s lab developed split cytosine and adenine base editors that can be reconstituted in vivo ...overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive...via FRET. Image from Beck and Gong, 2019. Broadening the spectral diversity of GEVIs can enable resolving...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...other antibodies in the experiment. If you are planning to use multiple antibodies in a single experiment...independent antibody strategies that compare the staining patterns of multiple antibodies against the same...express a tagged version of the antigen and compare staining of the antibody to that of the anti-tag antibody...negative controls as you get your assays up and running.  Ed.'s note: More questions on antibody validation...

...permanently or temporarily, we’ve got you covered. Defining your control requirements To pick the best tool...permanent control mechanism? Where is the control happening? Is the regulation of an exogenously introduced... Turn it on forever Permanently increasing or turning on expression of a new protein is a cinch. Stably...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...which Susan replied, “What’s a science writer?” Learning about SciComm by doing SciComm Writing had always... terms of PR types of things or communications planning.” To beef up her resume, she decided to complete...mind process things. Sometimes finding that uninterrupted time to be creative is challenging,” says Susan...Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking. Being...

...withdrawn from the US market because of life-threatening health effects. Eight of these posed greater ... important to get the research right from the beginning. This is the goal of Gendered Innovations. This...biological variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on ...compared to that of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies...

...photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab ...as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang...channelrhodopsin-2, commonly used in optogenetics, opening up the possibility of adapting GFP lines for optogenetics...Listen to Our Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,...When using the ClonTracer system, lentiviruses containing these barcodes infect the target cell population...infected a non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...