We narrowed to 658 results for: otos

1. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   ...of the most complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post,...

2. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   ...ligands, including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades...

3. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   ...position of having robust, null findings that are notoriously difficult to publish.  With Registered Reports...

5. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...

6. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than ...

7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   ...significantly stronger phenotypes than standard RNAi protocols without the need for mutant genetic backgrounds...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...

9. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...with the automation team to discuss automated protocols. 12:00 PM--Catch up. Finish any communications...

10. AAV Q&A with Tim Miles

    Type

    Blog Post

    ...A: At CLOVER, we have traditionally used qPCR (protocol link). We have found this method to be reliable...

11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...

12. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    ...Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...

13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...

14. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    ... (GEF-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock...

15. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    ...Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find Our List of CRISPR...

16. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ... CRISPR 101: Homology Directed Repair CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines ...

17. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ... one time. Weissman’s group has made standard protocols available and they are working to further optimize...

18. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    ...better understand developmental, metabolic, and photosynthetic processes. Often the tools developed for plant...

19. All in a Twist: dsRNA

    Type

    Blog Post

    ... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

20. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...observations about the issue, assessed potential protocol modifications, and ultimately decided on the changes...