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We narrowed to 509 results for: plasmid dna

Showing: 461 - 480 of 509 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...nonprofit that would facilitate the sharing of plasmids between scientists. The service would allow people... our first major repository milestone — 10,000 plasmids deposited and available — and nearly seven before...possible for scientists to both deposit and request plasmids, was a major focus of our first decade. We did...work In March 2015, Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see...also made us think of all the depositors whose plasmids we were entrusted with. So in June 2016, we launched...

  2. Plant Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...resources: Find plasmids for use in plants. Plasmid... Background Addgene’s repository contains many plasmid tools that were specifically designed for use in...use in plants. The tables below highlight plasmids spanning a wide variety of molecular biology technologies...fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific...

  3. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

  5. Bacterial Expression Systems

    Type
    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression ...CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR Plasmids page ...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Fusions Plasmids 101: Secondary Nanobody Toolbox Resources on Addgene.org View the Antibody plasmid collection...transcribed into cDNA fragments that are efficiently subcloned into the phagemid pDX_init plasmid, using the...The Seeger lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against...nanobodies can be synthetically expressed off of plasmids in bacteria, and they can target epitopes that...form of mRNA for academic research. The template plasmids for generating your own libraries are also available...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds... subcloned into pBXNPH3 or pBXNPHM3 expression plasmids for the production of tag-free proteins for further...

  7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi...

  9. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...you want to give GeoCas9 a try, as always, the plasmids are available from Addgene. Happy CRISPRing! ...Background with Addgene’s CRISPR Guide Page Find CRISPR plasmids at Addgene ...However, the recent discovery of GeoCas9 by the Doudna lab has opened up the field of thermophilic bacteria...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...Pre-constructed pairs of TALEN plasmids targeting zebrafish genes....TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...

  11. Genetic Code Expansion

    Type
    Collection
    ...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ...activity of a protein (e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate the ...Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All...

  12. COVID-19 Resources

    Type
    Collection
    ...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered...Bioworks to distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression...available Ginkgo SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV...

  13. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:...

  14. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell, 1987. 51...

  15. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene...

  17. Molecular Biology Reference

    Type
    Guide
    ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...fragment of DNA to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description...retain the plasmid DNA, despite the added replication burden, as bacteria without the plasmid DNA would not...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment...

  18. Cloning

    Type
    Guide
    ...ends in the DNA fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and... your DNA fragment of interest. Now that your DNA fragment has been cloned into a donor plasmid, it can...nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40...archaea, cleave double stranded DNA (dsDNA) at specific sequence sites in the DNA. In restriction cloning, scientists...end of dsDNA fragments to generate 3' single-stranded overhangs. DNA polymerases synthesize DNA molecules...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...any piece of DNA that already has restriction sites on either side of it into any plasmid that has the...

  19. CRISPR Guide

    Type
    Guide
    ...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...Browse Plasmids: RNA Targeting , RNA Editing Degrading DNA with Cas3 Rather than initiating DNA cleavage...-stranded DNA plasmid. Small ssDNA oligos often work well for small edits like a single-point mutation...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands are...

  20. Promoters

    Type
    Guide
    ...biology basics and plasmid components. Plasmids 101: The Promoter Region-Let's Go Plasmids 101: Inducible ...the binding of RNA polymerase to DNA. RNA polymerase transcribes DNA to mRNA which is ultimately translated...that the DNA tends to fold back on itself which helps to explain how many physically distant DNA sequences... popular Plasmids 101 series , covers topics ranging from the newest breakthroughs in plasmid technologies...Inducible Promoters Plasmids 101: Repressible Promoters Plasmids 101: Terminators and PolyA signals... Plasmid Reference Molecular Biology Reference Promoters...Promoters Promoters Definition A promoter is a region of DNA where transcription of a gene is initiated. Promoters...
Showing: 461 - 480 of 509 results