We narrowed to 610 results for: sam
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TypeBlog Post...fission yeast, Schizosaccharomyces pombe, have the same function as their counterparts in S. cerevisiae....
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Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...detected in cells electroporated with RNPs using the same guide. Nanoblades also had lower off-target effects... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting... -
6 Tips for Grant Writing
TypeBlog Post...institution). Likewise, if you’re staying in the same lab where you have been working for the last few... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...postdocs at institutions, will be challenging. At the same time, there is much opportunity to make headway ... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’s Ribobio... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...be at least 85% of the initial laser output. The same considerations apply to the polishing and preparation... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...data is freely available, and the collection of samples and sequencing is being organized and funded by... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for genome editing... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...one of its exons has simply been replaced with the same sequence flanked by LoxP sites while the resistance... -
Plasmids 101: Protein Expression
TypeBlog Post...protein expression. Plant systems share many of the same features and processing requirements as mammalian... -
Hot Plasmids: Summer 2025
TypeBlog Post...with TRV2 that encodes ISYmu1 and an ωRNA under the same promoter, they introduced heritable genome edits... -
Hot Plasmids: Winter 2025
TypeBlog Post...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...using Cas9, two or more gRNAs must be used along the same chromosome. During DNA repair, there is a chance...