We narrowed to 603 results for: sam

1. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   ...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...

2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   ... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with...

3. 6 Tips for Grant Writing

   Type

   Blog Post

   ...institution). Likewise, if you’re staying in the same lab where you have been working for the last few...

4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   ...postdocs at institutions, will be challenging. At the same time, there is much opportunity to make headway ...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   ...from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’s Ribobio...

6. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   ...be at least 85% of the initial laser output. The same considerations apply to the polishing and preparation...

7. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   ...data is freely available, and the collection of samples and sequencing is being organized and funded by...

8. Plasmids 101: Protein Expression

   Type

   Blog Post

   ...protein expression. Plant systems share many of the same features and processing requirements as mammalian...

9. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   ...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents...

10. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    ...2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for genome editing...

11. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    ...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ...

12. Hot Plasmids: Winter 2025

    Type

    Blog Post

    ...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...

13. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    ...using Cas9, two or more gRNAs must be used along the same chromosome. During DNA repair, there is a chance...

14. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...the general concept of the system has remained the same. An obvious drawback of the system is that it requires...

15. PhD Applications After COVID

    Type

    Blog Post

    ...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...

16. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...can differentiate into multiple cell types of the same lineage. Blood (hematopoietic) stem cells are a ...

17. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    ...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers...

18. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    ...one of its exons has simply been replaced with the same sequence flanked by LoxP sites while the resistance...

19. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    ... to deliver NgAgo ingredients into the cell, the same method used by Gao et al, 2016 (1). View the Complete...

20. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    ...Enrichment or depletion of multiple gRNAs targeting the same gene can be strong evidence that a particular gene...