We narrowed to 610 results for: sam

1. Plasmids for Endogenous Gene Tagging in Human Cells

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   ... For an N-terminally tagged protein, we used the same strategy, inserting the tag and the linker preceding...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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   ...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...detected in cells electroporated with RNPs using the same guide. Nanoblades also had lower off-target effects...

4. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical...

5. Making CRISPR Plasmids Using Fragmid

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   ...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting...

6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with...

7. 6 Tips for Grant Writing

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   Blog Post

   ...institution). Likewise, if you’re staying in the same lab where you have been working for the last few...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   ...postdocs at institutions, will be challenging. At the same time, there is much opportunity to make headway ...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’s Ribobio...

10. The Materials Science of Optogenetics Experiments

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    ...be at least 85% of the initial laser output. The same considerations apply to the polishing and preparation...

11. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...data is freely available, and the collection of samples and sequencing is being organized and funded by...

12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...and 2) gRNAs Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells ...

13. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers...

14. Plasmids 101: Protein Expression

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    ...protein expression. Plant systems share many of the same features and processing requirements as mammalian...

15. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...and look at them under a microscope, fractionate samples to isolate particular organelles and their contents...

16. Truncated gRNAs for Regulating Gene Expression

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    ...2015): 823-826. PubMed PMID: 26167643. 3. Kiani, Samira, et al. "Cas9 gRNA engineering for genome editing...

17. Hot Plasmids: Summer 2025

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    ...with TRV2 that encodes ISYmu1 and an ωRNA under the same promoter, they introduced heritable genome edits...

18. Hot Plasmids: Winter 2025

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    ...recombinant antibody in human postmortem brain samples. Results show positive staining in tissues from...

19. Degrading DNA with Cascade-Cas3

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    ...using Cas9, two or more gRNAs must be used along the same chromosome. During DNA repair, there is a chance...

20. Plasmids 101: Degron Tags

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    ...the general concept of the system has remained the same. An obvious drawback of the system is that it requires...