We narrowed to 734 results for: son
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TypeBlog Post...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260...
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Viral Vectors 101: Optogenetic Tools
TypeBlog Post... J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan... Jason Nasse... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., Wilson, J. M., & Sweeney, H. L. (2008). Adeno-Associated...Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-D., Bailey, C. G., & Rasko... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light...calcium release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...surface targets by tumor cells, are a few of the reasons antibodies may result in a lack of response. Antibodies...efficient immunotherapy must be to reach a flexible personalized treatment, and not universal correction of cancer... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes ... Darzi, M., Tarahomi, M., & Ghods, R. (2011). Comparison of the Photobleaching and Photostability Traits... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ...s41587-021-00938-z Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...a major impact. For example, you were the first person to identify a novel protein found in skin cancer...peer reviewer needs to be addressed to your own personal email directly. EB-1B outstanding researcher or... -
Antibody Guide
TypeGuide...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ... -
Chemogenetics Guide
TypeGuide...19607790 (Link opens in a new window) Atasoy D, Sternson SM (2018). Chemogenetic Tools for Causal Cellular...Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson SM (2011). Chemical and genetic engineering of...Ramirez MH, Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research...6. PMID: 11402033 (Link opens in a new window) Sternson SM, Roth BL (2014). Chemogenetic tools to interrogate...26399324 (Link opens in a new window) Vardy E, Robinson JE, Li C, Olsen RHJ, DiBerto JF, et al. (2015)... Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, You Z, Wu Z, Xi J, Haberman A, Graham... -
Adenovirus Guide
TypeGuide... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may... Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization...Asiedu, C., Geisbert, J., Stanley, D., Honko, A., Johnson, J., Mulangu, S., Pau, M. G., Custers, J., Vellinga...gene and cell-based therapies in the new era of personalized medicine . Genes & Diseases, 4 (2), 43–63. https...window) Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., Sharff, K. A., Luu, H. H., Haydon... -
Gamma-Retroviral Vector Guide
TypeGuide... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...Ravin, S. S., Su, L., Theobald, N., Choi, U., Macpherson, J. L., Poidinger, M., Symonds, G., Pond, S. ...Ridder, J., Allahyar, A., Wang, W., Carpenter, E., Mason, A., Wong, G. K., Cameron, E. R., Kilbey, A., & ...Srivastava, S., Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . .... -
Plan Your Experiment
TypeGuide.... D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J....PMID: 35941224 Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H...s41594-023-01090-9 PMID: 37783853 Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. ... -
Lentiviral Vector Guide
TypeGuide...third-generation packaging plasmids. For a summarized comparison of second- and third-generation systems, please... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836 Johnson, N. M., Alvarado, A. F., Moffatt, T. N., Edavettal... -
New Antibody Animation!
TypeBlog Post... Rachel Leeson... -
New Coomassie Protocol Video
TypeBlog Post... Rachel Leeson... -
New Western Blot Protocol Video!
TypeBlog Post... Rachel Leeson... -
New Antibodies in the Lab Animation!
TypeBlog Post... Rachel Leeson... -
Plasmids 101 eBook 4th Edition
TypeBlog Post... Rachel Leeson... -
Addgene Launches Partnership with IPI
TypeBlog Post... Rachel Leeson...