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We narrowed to 993 results for: Des

Showing: 481 - 500 of 993 results

  1. Science as Art

    Type
    Blog Post
    ...of familiar flowers." This new x-ray technique provides scientist with a new method to explore the inner...of delicate plants and other organisms without destroying the original, which could provide a new tool ... collected by Darwin on his voyage. Previous destructive techniques were incompatible with the need to...

  2. Genetic Code Expansion

    Type
    Collection
    ...based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past two decades, biologists have...included. For any and all questions you have while designing, implementing, or troubleshooting GCE experiments...non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites changes to UAG...

  3. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors... And the dataset is rich. Experimental data includes reagent type, target species, target name, host... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

  4. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...it relates to other teams at the company. This provides new (and old) employees with information on how...

  5. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent proteins... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

  6. An Addgene Summer Internship

    Type
    Blog Post
    ...instrument. They are the oldest sibling in a Guatemalan family of Mayan descent.   ...teaching me the basics of physics and sustainable design while allowing me to do my internship in the daytime...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396...

  9. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate...protein signal transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...packaged, when the vector is expressed. Once the design and construction steps are complete, it’s time ...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

  11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...blocks of polymers are heated up and pulled to a desired thickness. The fibers are drawn with microelectronics...whistles’ – including microscale light emitting diodes, thermal sensors, and microfluidic channels. These...using NeuroStack and the fibers as previously described, then measured electrophysiological potential ...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    .... MBP has a similar function as GST in that it provides a method to express a foreign protein in bacteria...protease. The carboxyl-terminus of the fusion protein includes an AviTag and a 6X HisTag for affinity purification...biotinylation and purification strategy outlined here provides a basis to determine binding kinetics using a ...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...supporting hybridoma sharing within the community for decades. Educational Resources The Basics of Western Blotting...

  14. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

  15. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ... to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different...untreated and sucrose treated viral preparations.    Despite observing a positive effect on our overall yield...due to impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and...

  16. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...time working through the details of experimental design and data with authors and referees. And I am constantly...visualization of science? Look at our graphics and design editors. How about condensing a week’s worth of...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

  18. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ... the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C....America. To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene...injected 60-80 animals per gene, they obtained the desired insertions on the first attempt for 6/7 genes. ...

  19. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While...a disease in a model system. TF screens can be designed to identify custom TF programs to acutely initiate...

  20. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...distribution of cell lines from repositories on opposite sides of the world shows that science is truly a global... et al., 2006), the hACE2 transgenic mice were designed after it was discovered that SARS-CoV, which entered...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ...
Showing: 481 - 500 of 993 results