We narrowed to 707 results for: ESC;

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...

...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

...worries, I've summarized the three strategies described in this post in the graphic below - including ...

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

...candidate genes in TS iPSCs to identify whether any rescue cell migration and contraction defects. The lab...

...when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,...

... interactions in events that occur on a short timescale, such as during drug action or cell signaling....

...to receive deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in...

...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

...recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive...

...Addgene’s blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...

...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...