We narrowed to 637 results for: ESP

1. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   ...Read the associated prompt carefully, plan your response, and let your inner wordsmith shine through. The...

2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   ...sequence just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't ...

3. New and Upcoming Viral Vectors - May 2020

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   Blog Post

   ...together encode the N and C terminal segments respectively of a GFP dependent Cre. The two segments come...

4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...they actually are. Throwing out plates that don’t respond as expected, use of representative images, measuring...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   ...reversibly bind and release a protein of interest in response to light allows for tagless protein purification...

6. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...practices suggested here may seem tedious at first, especially since you’ll want to dig in to the computation...

7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12 kb range. REPLACR-mutagenesis...

8. Plasmids 101: Blue-white Screening

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   Blog Post

   ...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly...

10. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ... of the technology to the public in an open, responsible manner. At Revolution Bio, we are addressing ...

11. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...kit also has other unique aspects that make it especially well-suited for use in stem cells. (See the complete...

12. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ...is characterized by point mutations in the Iron Response Element (IRE) RNA motif of the ferritin light ...

13. dTAG - You're it!

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    Blog Post

    ...recent papers noted below for examples of dose-responses and time-courses (Nabet et al., Erb et al., Huang...

14. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    ... chicken IgY domains. Chicken IgY domains are especially useful for multi-color staining of tissue because...

15. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...say the journal club was a rousing success–the response was a full sixty seconds of silence–but it was...

16. To Each HIS Own

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    Blog Post

    ... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

17. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...Tetracycline-dependent promoters are engineered by placing tet response elements upstream of a minimal promoter. These...

18. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...

19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...are established monomeric and dimerizing FPs respectively, are used as positive and negative controls ...

20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...