We narrowed to 639 results for: ESP

1. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...scientists with me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna...

2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing is robust, with...

3. Plasmids 101: Inducible Promoters

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   Blog Post

   ...normally inactive and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and...

4. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...extinction coefficient (EC) and quantum yield (QY) respectively. The theoretical brightness is calculated by...

5. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...services. There was an overwhelmingly positive response to the option of Addgene providing plasmids in...

6. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   ...practices suggested here may seem tedious at first, especially since you’ll want to dig in to the computation...

7. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...plasmids up to 25 kb, most cloners should be fine, especially using plasmids in the 10-12 kb range. REPLACR-mutagenesis...

8. Plasmids 101: Blue-white Screening

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   Blog Post

   ...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...of PTG1 and PTG2 were 3 and 31 times higher, respectively. In rice protoplasts, Xie et. al observed slightly...

10. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ... of the technology to the public in an open, responsible manner. At Revolution Bio, we are addressing ...

11. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...kit also has other unique aspects that make it especially well-suited for use in stem cells. (See the complete...

12. Antibodies 101: Conjugation

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    Blog Post

    ...cover all of the possible antibody conjugates (especially if you start to consider clinical applications...

13. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...say the journal club was a rousing success–the response was a full sixty seconds of silence–but it was...

14. To Each HIS Own

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    Blog Post

    ... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

15. Plasmids 101: The protein expression toolbox

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    Blog Post

    ...Tetracycline-dependent promoters are engineered by placing tet response elements upstream of a minimal promoter. These...

16. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...triphosphate in the gRNA, reduce the innate immune response associated with gRNA introduction (Allen, et al...

17. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...from a vendor that you use frequently, with a responsive and knowledgeable technical support team, is ...

18. What's New in CRISPR - November 2021

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    Blog Post

    ... particles for delivery into mammalian cells, especially when fused to effector proteins for gene activation...

19. RNA Extraction Without A Kit

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    Blog Post

    ...Furthermore, RNases, or enzymes that degrade RNA, are especially hardy proteins that are found in and on everything...

20. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...gut can provide insight into how the microbiome responds to infection, dysbiosis, and antibiotic treatment...