We narrowed to 672 results for: FIE

1. Fluorescent Biosensors for Measuring Autophagic Flux

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   Blog Post

   ...every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine...

2. Science Careers: Unruly Interests Feed Many Paths

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   Blog Post

   ...unusual job openings, although they seemed too far afield at first and I let them pass by. But I didn’t forget...

3. Plasmids 101: Origin of Replication

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   ...die off. Have any questions? Need something clarified? If so please, post in the comments.  References...

4. Meet the New Addgene Board Members!

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   ...solutions to crises like those and ended up in the field of gene therapy.” He’s also worked on vaccines throughout...

5. Summer SciComm Series: Master of SciComm

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   ...career in science communication, an established field of inquiry with which I was not yet familiar. The...

6. Addgene Begins Distribution of Recombinant Antibodies

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   ..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...

7. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   ... proteins with 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection...

8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   Blog Post

   ...vectors are viruses that have been genetically modified to either limit or completely eliminate their ...

9. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   ...pseudovirus neutralization assay. Neutralization was quantified in terms of luminescence. Image from Garcia-Beltran...

10. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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    Blog Post

    ...article.) Modifying AraC to create BLADE The team modified AraC to create BLADE by replacing its dimerization...

11. 10 Great Guest Posts We're Thankful For

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    ...uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable functions...

12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post

    ...dCas9 regulatory switch. By no means limited to the field of metabolic engineering, this is such a powerful...

13. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post

    ...learned, especially noting action items that are identified. Keep a journal to record topics, plans, findings...

14. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post

    ...-color stimulation has been a holy grail of the field. The problem is that all of these molecules are ...

15. Colony Formation Titering Assay

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    Protocol

    ...produces a lawn of cells, which cannot be accurately quantified. Add 150 μL of a viral dilution to each well ...

16. FPbase: A new community-editable fluorescent protein database

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    Blog Post

    ...more powerful ways, and the advanced search page simplifies the process of building advanced multi-parametric...

17. Celebrate National Book Month with Addgene

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    Blog Post

    ... would also be a great way to level the playing field for our scientists and non-scientists. It wouldn...

18. New and Upcoming Viral Vectors - September 2019

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    ...populations with intersectional approaches is a growing field that we aim to support by growing our inventory ...

19. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...when studying neuronal activity. The researchers identified MerMAIDS from their analysis of different publicly...

20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    ...of apoptosis in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 ...