We narrowed to 877 results for: HA

...the opsin you use); the efficacy of optogenetics has already been proven. Therefore, a concrete, well ...scientists who pioneered the development of optogenetics, has genetically manipulated a number of the opsin genes...kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic constructs...stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs with various...

...RNA and can express fluorescent proteins. This lab has created an automated tank for feeding and maintenance...scolopes (Joshua Rosenthal) The Hawaiian bobtail squid has an extreme number of nerve cells and an unusually... injection (hard to do, expensive, not every lab has the capability) with receptor mediated endocytosis...

... improve its stability in vivo. However, Spinach has proven useful in the engineering of riboswitches....syndrome (Strack et al., 2013). Since then, Spinach2 has been used to investigate intracellular viral DNA ...better choice for imaging than the monomer. Broccoli has been developed into a split aptamer that can be used...

...stringently controlled and has a low copy number. Like the E. coli chromosome, it has a single ori and requires...Once the average copy number for a given plasmid has been reached, negative feedback circuits reduce replication...

... and allows targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As ..., the search for smaller, compact genome editors has become a key focus in recent years. TnpB nucleases...nuclease domains (HNH and RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single...

... newest kid on the block in the sequencing world has been long-read sequencing. Whereas the individual...auto-alignment, plus additional automation in the backend, has enabled the QC to keep up with the pace of deposits...plasmid. By working with the Data team, the QC team has also improved efficiency in many of their methods...

...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...emotions, and even what therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes... pretty biased as the founder, but our book club has become one of my favorite parts of Addgene’s culture...

... an assistant professor at Kyushu University. He has a particular interest in the development of sensory...Imai is a professor at Kyushu University. His lab has been studying functional and anatomical aspects of...

... have been spent within a lovable community that has allowed me to expand my knowledge more than ever ... had opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want...

...cells, but since the majority of the viral genome has been swapped for alternative genetic cargo, they ... there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for...

...requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify...in human pluripotent stem cells, a cell type that has been difficult to modify in the past.   Figure...

...depositing lab. If your vector is large (>10 kb) or has a low copy number, try following protocols for low...easiest and clearest ways to determine if your sample has recombined. Choose a restriction enzyme that will...

... A practical guide by Paula J. Silvia  This book has tips for how to write a lot as an academic. If you...your Mendeley library in Microsoft Word. Mendeley has a web and desktop version that works with Windows...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

... to objects that exist online, but this approach has been adapted for the identification of physical materials...Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier...

...TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So how does...PCR products. Keep in mind that Taq polymerase has an error rate of about 1 in 3,500 bases. Typically...

...library screen; instead I thought they would focus on “Has this person been consistent throughout their career...division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you ...

...prokaryotes; the ultimate victor will be the species who has the strongest will to survive. Genome engineering...biological and translative technologies. Addgene has empowered researchers to harness previous experimental...

...restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...