... receiving training modules in synthetic biology has grown into a recognized name in the field of STEM...logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder book chapter and an ...

...bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study ...

...supernatant during the production phase. However, this has not been well characterized as it is not a common...microvesicles as a vehicle for drug delivery that has recently gained interest special contexts, such as...

...used. EvolvR: key components The EvolvR system has two key components: the error prone PolI3M that’s...BioRender.com. The initial version of EvolvR has an editing window of ~17 nt from the nick site, which...

...slower, more traditional, QC process. While this has worked well for the early sets of SARS-CoV-2 materials...certain processes in both core operations. As it has been in much of the world, we have found ourselves...

...time Since the publication of our paper, our group has been inundated with requests to share our plasmids...). Also, we’ve got to mention that our FedEx guy has been the best: Thanks, Todd! As you can imagine, ...

... size distribution and concentration of ApoB-LPs has been shown to have a strong correlation with cardiovascular...indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in ...

...with the indicated function. One huge reason CRISPR has become such a popular genome editing tool is its ...next-generation sequencing to ensure that the library has retained gRNA representation, produce high titer ...

...sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria and produces...ordered directly from Addgene. The Podgorski Lab has provided a brief breakdown of the promoter systems...

...neurons, directs high-level transgene expression, and has retrograde functionality to monitor and map neurons...doors in neuroscience. The retrograde serotype also has promising therapeutic applications; it can enhance...

...targeting, where siRNAs are ineffective and Cas13 has steep off-target costs. Additionally, this system...produced at Addgene and, as you might suspect, it has been one of our most commonly requested antibodies...

...HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...yeast that same day.  The field of synthetic biology has led to advances in biofuel generation, green chemistry...

... chosen promoter, and Bloomington’s Stock Center has fly lines expressing Gal4 under a variety of promoters...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification.... Using the same example plasmid as above (which has the XbaI site blocked by methylation), the figure...

...disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged behind...

...reagents. F1000Research, among many other journals, has joined a pilot project from the Neuroscience Information... up their own minds on whether the data analysis has been of good quality and whether they agree with ...

...and the master plasmid in one step. The kit also has other unique aspects that make it especially well-suited...for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be more efficient...

...restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...

...turning on and off the light source. Optogenetics has been broadly applied to study the physiology of the...channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance at physiological pH and trafficks...Depolarizing) ChR Variants. The optogenetic toolbox has been expanded by scientists through both the identification...heterodimerization can reconstitute the protein. This principle has also permitted the design of synthetic two-part transcription...

...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...