We narrowed to 526 results for: MEA;

1. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   ...low dark-state activity and toxicity. All of that means this light-powered sword truly is an elegant tool...

2. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   ...polymerase II promoters. The design of these plasmids is meant to more closely mimic pre-miRNAs, and the use of...

3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...titering IDLVs, there is no integrated piece to measure! Instead, two commonly employed methods for titering...

4. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ...advantages are rooted in their size. Being smaller means scFvs are easier and cheaper to make because they...

5. Multiple Plasmids at a Low Price: Kits from Addgene

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   Blog Post

   ...sites used in these vectors are of low abundance (meaning they are unlikely to be found in your gene of interest...

6. Site Directed Mutagenesis by PCR

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   Blog Post

   ...resistance marker from the parental plasmid provides a mean for selecting for transformants which have taken...

7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   ... the percentage of cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell...

8. Gamma-Retroviral Vector Guide

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   Guide

   ...only infect mitotically active cell types. This means that lentiviruses can infect a greater variety of...

9. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   ...failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders...

10. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...activity or gene expression. Experimental output is measured and quantified. Created with BioRender.com. ...

11. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...will cleave DNA frequently enough to result in a smear of fragments that get smaller in size as a function...

12. Finding Your Perfect Job After University

    Type

    Blog Post

    ...citizen, I was entitled to a Work-Travel VISA, which means I can gain employment for up to 6 months in Australia...

13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    ...should not be confined by birth country or financial means. Instead, talent should be realized through equal...

14. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...and your development. Taken together, all of this means that you need to introduce yourself to and talk ...

15. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...achieve exponential amplification. What does fidelity mean for a ligase? DNA ligases generally prefer fully...

16. Important Considerations When Using AAVs

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    Blog Post

    ...Modified from Aschauer et al. (2013) What does this mean to us? Well, an AAV user must determine which serotype...

17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    ...as their selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of...

18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    ...plasmid amplification (Fischer, KB. 2019). This means that all DIO/FLEx AAV vectors will have some level...

19. Transferable Skills Guide: Teamwork

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    Blog Post

    ... all team members understand what roles they’re meant to fill and what their responsibilities are. That...

20. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    ...the substrate with the target DNA sequence. This means cloning occurs in vivo, as compared to restriction...