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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Mos

Showing: 481 - 500 of 772 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

  7. CRISPR Guide

    Type
    Guide
    ...Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu...inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target...simplicity and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...ease of generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR...mutations (see: Validate genetic modification ). In most cases, NHEJ gives rise to small indels in the target...homology elsewhere in the genome. Realistically, most gRNA targeting sequences will have additional sites...specificity. SpCas9 (from Streptococcus pyogenes ) is the most popular Cas endonuclease, with many modified versions...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...
Showing: 481 - 500 of 772 results