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We narrowed to 765 results for: Mos

Showing: 481 - 500 of 765 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

  7. Molecular Biology Reference

    Type
    Guide
    ... from chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant...conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...relationship with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid. These tools are essential to the...recombinant DNA Molecular Cloning Plasmids that are used most commonly in the field of recombinant DNA technology...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

  9. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

  10. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

  14. Antibody Guide

    Type
    Guide
    ...immune system’s response to foreign bodies, are the most commonly used affinity reagents. Antibodies are ...against the antigen, packaged, and sold. This is the most cost-efficient way to produce antibodies, with the... IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer with...anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found in tissues...Produced during the primary immune response; found mostly in blood Pentamer with a valency of 10 Antigen ... for exact storage requirements. As a guideline, most antibodies can be stored at 4 °C for short-term ...allow detection of the antibody:protein complex. The most commonly used types of signaling molecules are horseradish...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

  16. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...
Showing: 481 - 500 of 765 results