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We narrowed to 876 results for: NSI;

Showing: 481 - 500 of 876 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... to solve the problematically low fluorescent intensity of previous versions of Brainbow. Developed from...this system useful for visualization of cellular extensions, such as neurons in the fly visual system, which...cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation of mature pigmented melanocytes...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...donor (e.g., sister chromatid, plasmid, etc.), and consists of three pathways: classical double-strand break...you need to generate a knock-in.   General considerations for designing a repair template to create mutations...cells than their plasmid counterparts. Another consideration for dsDNA templates is their ability to randomly...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...material, which ensures the editor will only be transiently present in transduced cells, limiting off-target.... (2024). Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes...CRISPRoff system — a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...retroviral backbones. On some occasions, shRNAs are transiently expressed using either AAV backbones or species-specific...synthetic, mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro...time you need to knock down a gene, maybe you’ll consider using RNAi! References and Resources References...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...to be examined on a case-by-case basis. GFP is sensitive to acid The chromophore of GFP can exist in a ...determined in vitro by measuring the fluorescence intensity of purified protein at different acid concentrations... its fluorescence and therefore GFP cannot be downsized by deleting residues. Only about 10 amino acids...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... social science to an audience that I thought consisted of all biologists. The social scientists in the...a solution or overcame obstacles. Take time to consider the order of your narrative. Scientists do this...are worried that this might sound condescending, consider explaining the term on a slide but not reading...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...repetitive sequences, but if present, a minor extension can usually ensure that the 3’-base(s) do not ...manufacturers’ PCR protocol. Be sure to adjust the extension time to match the size of your template. Amplification...PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ...industry positions. There were, however, some downsides to working in a large commercial company. In my...sometimes go unnoticed at large organizations. Another downside to large commercial companies which are reliant... treatment outcomes. I would say that the only downside of modern genetics from the perspective of somebody...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...professional training after earning a doctorate Considered a "stepping stone" position to become a professor... lab Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware...1-2 years) Often a gap-year position taken to transition to the next career stage (ex: grad school, med...

  12. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...steps 2-4 25-30 times. Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding...below the primer melting temperature (Tm). Set extension step at 1-2 minutes per kilobase of product depending... instructions for specific instructions about extension time and temperatures. Initial Denaturation for...°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. Run 2 μL on a gel to check...The rate of DNA synthesis is ~1-2 kb/min. The extension time can be adjusted according to the length of... temperature around 70°C and is the molecule responsible for driving the DNA synthesis. Sterile dH 2 O...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...the surface or the inside of your cells. While proteins themselves can emit intrinsic fluorescence when...fixable dye stains that react with free amines on and inside the cells and are thus typically used for distinguishing...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...sequences can be optimized for specificity and sensitivity, for example by changing certain amino acids ... that can pass through each well - the optical density (OD) - of the known and experimental wells is measured...is used to detect proteins on the surface of or inside whole cells. Antibodies - typically, primary antibodies...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...developed in David Liu’s lab in 2019, has added considerably more precision and flexibility to the CRISPR...engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to a reverse transcriptase...mismatch repair   ✓   ✓ ~ ~ ~ Transiently co-express mismatch repair inhibitor MLH1dn ...

  17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...culture of bacteria transformed with your plasmid Resuspension buffer Denaturing solution Renaturing solution... This step gets all of the bacteria back into suspension, but within a smaller volume of buffer that is...Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH 8) 50 mM glucose 10...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase... mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension can take time, it is a good idea to let it sit...eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference Page Top Index...

  18. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...monoclonal line could be generated at any time. Considerations Before You Start Some cell lines tend not to...optimized for adherent cells but can be modified for suspension cells. The number of monoclonal lines generated... this, you are seeding the plate at an average density of 0.5 cells/well. Seeding an average of 0.5 cells...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9... in individual wells of a 96-well plate after expansion of single cells for 13 days. Note that growth ...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  19. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...liquid culture is capable of supporting a higher density of bacteria and is used to grow up sufficient numbers...need to grow for a longer time to get the correct density of bacteria since they will grow more slowly at...specific protocol and conduct an OD600 to measure the density of your culture if needed. A good negative control...culture. More aeration may help to increase the density of the culture. Normally cultures shake at 150 ... this to 350 - 400 rpm to obtain a higher cell density. Reference Page Top Index...

  20. Cloning

    Type
    Guide
    ...vectors and recombination enzymes can be quite expensive. In addition it is quite difficult to switch to...
Showing: 481 - 500 of 876 results