We narrowed to 588 results for: RET

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   Blog Post

   ...wanted to get her PhD. “Going back to school was a pretty easy choice for me,” she said. “I had always been...

2. Plasmids 101: Terminators and PolyA signals

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   ...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID...

3. The Materials Science of Optogenetics Experiments

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   ...inconsistent, and behavioral results impossible to interpret. Polishing the fiber optics with diamond polishing...

4. Experimenting in an Open Source lab: from CRISPR to Cats

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   ...Microsoft Research working on speech recognition, he returned to school to merge his interests in biology and...

5. Degrading DNA with Cascade-Cas3

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   ...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...With a few extra lines of code, we enabled it to return guides and primers in batch, optimized for HDR....

7. Transferable Skills Guide: Managing a Team

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   ... realized that at the time I could likely have retained this talented undergrad. The point here is managing...

8. Google Forums Round Up: First Impressions of NgAgo

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   ...NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO DEMONSTRATE...

9. Plasmids 101: Optimizing Plasmid Yields

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   ... in a protein expression strain, we suggest retransforming the plasmid into a strain meant for DNA purification...

10. 3D Printing Meets CRISPR Cas9

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    ... is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily forgetting...

11. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One way to...

12. Viral Vectors 101: Producing Your rAAV

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    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

13. Plasmids 101: Degron Tags

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    ...addition of a small molecule, and protein levels can return to normal once the drug has been removed. The degradation...

14. The Scientific Conference Poster Session: Tips for Success

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    ...your poster on your computer screen than it is to pretend you’re standing at the session. Present with confidence...

15. Polymerase Chain Reaction Overview and Applications

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    ...not as quantitative as other methods of PCR. Theoretically scientists should be able to determine the quantity...

16. Experimenting with New Careers while in Grad School

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    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...

17. PITChing MMEJ as an Alternative Route for Gene Editing

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    ... the region between the microhomology and the retention of a single microhomology sequence. For more mechanistic...

18. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...easy Very hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing ...

19. Genome-wide Screening Using CRISPR

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    ... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...

20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...recently to explore their use in plants, including FRET tools, double dimerization GFPs, and circular permutation...