We narrowed to 609 results for: SHE

1. OCaMP: A New Calcium Indicator for Neural Imaging

   Type

   Blog Post

   ...: Zoomed-in view of traces corresponding to the dashed box above. The number of action potential (AP) ...

2. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   ... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...

3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...

4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   ...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...

6. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   ...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...

8. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

9. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ...moment you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to ...

10. Lentiviral Vector Guide

    Type

    Guide

    ... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ...

11. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...Serotype refers to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow...

12. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    ...Your video conferencing software freezes and then crashes. You’re muted on Zoom again.  These things don’...

13. The time and cost required to make a plasmid

    Type

    Blog Post

    ... PI or corresponding author of the paper it’s published in. That’s also a great way to get to know people...

14. To Each HIS Own

    Type

    Blog Post

    ...used for a variety of assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies...

15. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    ...and therefore each target protein. This can be accomplished by using a panel of primary antibodies conjugated...

16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain...

17. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ...PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization...

18. Antibodies 101: Conjugation

    Type

    Blog Post

    ...conjugated antibodies that are ready to use off the shelf. So why are we talking about this? Even though there...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    .... Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    ...Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional Resources on...