We narrowed to 542 results for: SHI

1. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...against the database. Another benefit is that a frameshift mutation present in the ORF is readily apparent...

2. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...them had imagined for a scientist. I think that establishing this history helped demonstrate that a) I was...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...levels. BiFC quantification This is where BiFC outshines FRET — BiFC experiments only require that you ...

4. Getting to Know Expansion Microscopy

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   ...they want to understand the ultrastructural relationships that influence everything from cell division...

5. CRISPR 101: Making a Knock-In Cell Line

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   ...is to disrupt DNA translation by generating a frameshift mutation. The majority of cellular repair events...

6. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...

7. A Guide to Starting Your Own Journal Club

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   ...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

8. Tips for arabidopsis transformation

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   ...are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can...

9. CRISPR Antimicrobials

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   ...phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics, but the field...

10. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...

11. Save Time with Transient Plant Leaf Transformations

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    ...precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...

12. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...groucho gene? Check out plasmids deposited by the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic...

13. Designing Your Chalk Talk for the Academic Job Interview

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    ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...

14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...animals are engineered by introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic...

15. Antibodies 101: Immunoprecipitation

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    ... the non-specific proteins are removed through washing, the target protein can be eluted. The elution ...

16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ...Panda, S., Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, Y., Nayak, A. K., Baig, M. J., & Molla...

17. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...the way. Make connections, and nurture those relationships. Then when you are ready to apply for your dream...

18. Career Insights: Technical Support Specialist

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    Blog Post

    ...the many individual stories and get (sometimes refreshing) insights into how other scientists think and...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...Hok translation is coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation...

20. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...22368488. PubMed Central PMCID: PMC3279232. 10. Song, Shiping, et al. “Aptamer-based biosensors.” TrAC Trends...