Addgene

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Showing: 481 - 500 of 576 results

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Adeno Associated Virus (AAV) for Cell and Gene Therapy

Type

Blog Post

... disease. To do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience...
Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

...underlying causes of this unreliability: cross-reactivity (binds to non-target proteins), variability (...
Split Fluorescent Proteins for Studying Protein-Protein Interactions

Type

Blog Post

...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow...
How-to: 5 Steps to a Great Panel Discussion

Type

Blog Post

...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...
Plasmids 101: Protein Expression

Type

Blog Post

...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...
Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...
PhD Applications After COVID

Type

Blog Post

... receiving applications, universities typically invite a select group of students to continue the application...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

Showing: 481 - 500 of 576 results

Results per page:

Adeno Associated Virus (AAV) for Cell and Gene Therapy

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Corralling and Cornering the Goldilocks Antibody

Split Fluorescent Proteins for Studying Protein-Protein Interactions

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

5 Tips for Troubleshooting Viral Transductions

How-to: 5 Steps to a Great Panel Discussion

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Tips for a 1st time AAV user (by a Rookie AAV user)

AAV Vector Quality Control: Going the Extra Mile with NGS

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Plasmids 101: 5 factors to help you choose the right cloning method

Plasmids 101: Protein Expression

Production of Virus in Insect Versus Mammalian Cells

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Twenty Years of Addgene Sharing: CRISPR

PhD Applications After COVID

PCR Applications for Copy Number Variation Assays

Polymerase Chain Reaction Overview and Applications

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