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...generally publish 5-10 Hot Plasmid articles with each of our newsletters. You can find all of these articles...you haven’t got time to sit down and read through each one, plug in some headphones to learn more about...through this podcast segment. Below, we break down each of the Hot Plasmids segments into its own (highly...

...tasked the students to separate out colonies from each individual strain using the streak plate technique...University of Science and Technology, has been teaching the microbiology lab every year for over 20 years...strains are easily amenable to other experiments to teach other aspects of molecular biology. “What I'd like...

...these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students about ...Working on Rare Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org...

...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...molecule, are often named after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer...

... is my pleasure to announce that we once again reached new heights on the Addgene blog: we surpassed 60,000...Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes a ...currently on my way back from a very successful Outreach Trip in San Francisco and would like to give my...

... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...available from Addgene as pools provided in two sets, each with 4 gRNAs per kinase, so screens can be performed...gRNA, for use with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as...

...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...et al., 2016), creating a collection of plasmids each carrying a single gene with a defined mutation or...the mutations to functional groups, based on how each one impacted cell viability (see figure). Most of...

...sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each set...Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant ...

...genomic deletions We were inspired to produce intrachromosomal deletions based on the experiments of Kim ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

...works better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled...code your Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists...

...Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each other, or are friends...this cluster analysis. The closer two dots are to each other, the more friends they have in common. Only...

...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...our website, which receives 3.8 million visitors each year.  This year, we are excited to launch our Antibody...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

... vectors contain two origins of replication, one each for E. coli and Agrobacterium, along with a selectable...size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication ...and codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency...

...representing the specific recombination site for each enzyme, were generated to test recombination efficiency...efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent RFP expression...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...

...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...contrast agents for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light...

...and Figshare but we are working on expanding our reach. We can’t save you the hard work of reading papers...ReFigure extension and visit ReFigure.org. You can reach the ReFigure team at [email protected] Please...Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete and open ...

... of affinity tags, the proteins they work best attached to, and the species they are compatible with are...nature of the size, structure, and other qualities of each tag class, they are generally best suited for their...101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...

...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

...proximity to an endogenous gene. Each trap uses a slightly different approach (described in detail below),...something species-specific) or a selectable marker. Each insertion line can then be isolated and screened...

...protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction sites with different...RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...