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We narrowed to 949 results for: ache

Showing: 481 - 500 of 949 results

  1. General Transfection

    Type
    Protocol
    ...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...ratio will need to be empirically determined for each new batch of 1 mg/mL PEI prepared. There may be ... used, volumes, pH adjustment etc. Consequently, each batch needs to be validated and the best ratio of...

  2. Protocol - Bacterial Transformation

    Type
    Protocol
    ...cell/DNA mixture on ice for 20-30 mins. Heat shock each transformation tube by placing the bottom 1/2 to...enough colonies for your next step. Remember that each of these shortcuts will reduce the efficiency of...cuvettes. Follow the manufacturer's instructions for each. I got no transformants. What went wrong? Check ...

  3. Immunocytochemistry

    Type
    Protocol
    ...Place a sterile poly-D-lysine coated coverslip in each well of a 24-well cell culture treated plate. Seed... aspirate the media from the 24-well plate. Wash each well with 500 µL of PBS, remove the wash, and dispose...dispose of it in an appropriate waste container. Fix each well with 500 µL of cold 4% paraformaldehyde in ...

  4. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...Scientists on Twitter. Each dot represents a scientist. Only scientists who follow each other, or are friends...this cluster analysis. The closer two dots are to each other, the more friends they have in common. Only...

  5. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...our website, which receives 3.8 million visitors each year.  This year, we are excited to launch our Antibody...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

  6. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ... vectors contain two origins of replication, one each for E. coli and Agrobacterium, along with a selectable...size, they shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication ...and codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...representing the specific recombination site for each enzyme, were generated to test recombination efficiency...efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent RFP expression...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...

  8. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher...contrast agents for two-color imaging in animals. The approach relies on ultrasound waves as opposed to light...

  10. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ... of affinity tags, the proteins they work best attached to, and the species they are compatible with are...nature of the size, structure, and other qualities of each tag class, they are generally best suited for their...101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...

  11. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...meetings, when Melina, our CSO and co-founder, approached me and asked about my current projects and personal...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

  12. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...proximity to an endogenous gene. Each trap uses a slightly different approach (described in detail below),...something species-specific) or a selectable marker. Each insertion line can then be isolated and screened...

  13. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI restriction sites with different...RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

  14. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...

  15. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...others in the field where to find it. A good way to reach others in the science blogosphere is to read and... great place to find opportunities for science outreach. Here in the North Carolina Research Triangle ...He got a little money for his trouble, too. 7)   Reach out to science writers in your area or at your institution...

  16. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don...protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder, Melina...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...

  17. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...focus on the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The DNA...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

  18. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

  19. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository...really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic Biology Engineering... database. Through a series of simulations and machine learning, they created a classifier that predicts...

  20. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which... bacteria refuse to grow. Eventual success was achieved by inserting BirA into a pMal plasmid (NEB) thus...
Showing: 481 - 500 of 949 results