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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 481 - 500 of 830 results

  1. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...

  2. Clear and Accessible Writing

    Type
    Blog Post
    ...writing applied to alt text. We also were aware of the need for clear, accessible writing in scientific writing...are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between the...

  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...like the right approach for your experiment, you’ll need to decide exactly which type of virus to use. Types... viral particles inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses...

  4. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... price. With this increased complexity comes the need (and/or opportunities) to modify the device for ...components are adapted and combined with creativity and feedback from the community to create state-of-the-art ...

  5. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....

  6. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...

  10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...

  11. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

  12. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  20. Cloning

    Type
    Guide
    ... there is an ideal approach. This may be due to speed, cost, availability of starting materials or just...compatible ends together. TOPO® cloning thus does not need restriction enzymes or an exogenous ligase providing... of PCR products into donor plasmids without the need for restriction enzyme cloning. For more information...of DNA in the chosen orientation, and without the need for any unwanted sequence at the junctions (such... a seamless manner. To accomplish this, you just need to introduce into the yeast the two (or more) fragments...would like to fuse. A major disadvantage is that you need to be set up to grow, transform and purify DNA from...
Showing: 481 - 500 of 830 results