We narrowed to 709 results for: esc

1. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   ...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ...Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they call CRISPR/Cas9...

3. Plasmids 101: Yeast Vectors

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   Blog Post

   ...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

4. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein degradation...

5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ...candidate genes in TS iPSCs to identify whether any rescue cell migration and contraction defects. The lab...

6. What's New in CRISPR - November 2021

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   Blog Post

   ...miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature Cas system that utilizes...

7. Transferable Skills Guide: Cross-team Communication

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   Blog Post

   ...when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,...

8. Summer SciComm: Preprints

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   Blog Post

   ...to receive deposits of plasmids that have been described in a preprint. In fact, over 1,500 plasmids in...

9. Summer SciComm Series: Cognitive Load

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   Blog Post

   ...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

10. Antibodies 101: Affinity Reagents

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    Blog Post

    ...recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive...

11. Antibodies 101: Secondary Antibodies

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    Blog Post

    ...Addgene’s blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction...

12. RaPID Detection of RNA-protein Interactions

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    Blog Post

    ... interactions in events that occur on a short timescale, such as during drug action or cell signaling....

13. The Future of Research Symposium Boston 2015

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    Blog Post

    ...driglar.     References 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws...

14. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...In early 2011, the Bogdanove and Voytas groups described a new Golden Gate-based technology for genome ...

15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...down your own results; are they the same as the description the authors give? Ask yourself if the results...

16. Plasmids 101: Cre-lox

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    Blog Post

    ... Combining this with some of the loxP methods described above, a genetic modification can be restricted...

17. Working with Nuclear Receptors

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    Blog Post

    ...cardiovascular disease, cholesterol gallstone disease, senescent illnesses, and the metabolic syndrome. Coregulators...

18. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ..., can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into ...

19. Rabies and Neuronal Tracing

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    Blog Post

    ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain...

20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...partners other than those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify...