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  1. Synthetic Biology - Worm

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    Collection
    ...Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic...

  2. CRISPR Guide

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    Guide
    ...that defines the genomic target to be modified. You can therefore change the genomic target of the Cas...of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-...not permanently modify the genomic DNA. dCas9 has also been used for genome-wide screens to activate or...visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify Genomic Regions ...RNAs), and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...associated with a genomic region of interest in vivo. Figure 12: Overview of purifying genomic regions using...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    ...Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...organisms in which Cas9 has been integrated into the genome. gRNA Empty Vectors Many gRNA empty vectors have...FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector Mammalian...promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome editing in plants Plant Plasmids for six gRNA module...System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants Plant PCR-based...Liu A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three...

  4. TREAT-AD Plasmid Collection

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    ...Plasmid Collection You may also like... Structural Genomics Consortium (SGC) Plasmids Opher Gileadi Lab plasmids...

  5. TALEN Guide

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    ...able to manipulate a specific region of DNA in the genome of your favorite species almost as easily as correcting...fingers, potential targetable sites in your average genome are every 500 bp. TAL effectors have some restrictions...

  7. Plan Your Experiment

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    Guide
    ...101: Validating your Genome Edit . Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...system that enables researchers to manipulate the genome like never before. This section will provide a ...particular gene(s) without permanently modifying the genome dCas9-repressor (such as dCas9-KRAB) or dCas9 gRNA...endogenous gene(s) without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting... be used in vivo A common choice for conducting genome-wide screens using CRISPR AAV transduction CRISPR...Addgene depositors JOVE Video Reprint: Protocol for genomic deletions in mammalian cell lines Select Your Target...design your gRNA. Know your cell line/organism and genomic sequence When possible, you should sequence the...

  8. Adeno-associated virus (AAV) Guide

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    Guide
    ... into the genome. This is important because local chromatin structure at the site of genome integration...preferred site of genomic integration on human chromosome 19, termed AAVS1. Instead, the rAAV genome is typically...References AAV Components The small (4.8 kb) ssDNA AAV genome consists of two open reading frames, Rep and Cap...available from Addgene. The popular CRISPR/Cas9 genome editing system has been modified for use with AAV...system represents a major step forward for in vivo genome editing. For more information on AAV-CRISPR, please...lysogenic cycle, wild-type AAV integrates into the host genome at a specific site, AAVS1 on human chromosome 19...AAV to integrate at non-homologous sites in the genome, and do so at about a 0.1% frequency. Nonetheless...

  13. Lentiviral Guide

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    Guide
    ...incorporated the lentiviral genome and harbor the genetic information encoded by that genome. Many lentiviral transfer...of the transfer plasmid sequences into the host genome. Typically it is the sequences between and including...including the LTRs that is integrated into the host genome upon viral transduction. Many lentiviral transfer...The graphic to the right shows how the lentiviral genome is edited down and distributed across the three...standard retroviruses. That is, many lentiviral genomes have selectable markers, such as the puromycin ...cells that have not incorporated the lentiviral genome and those cells that survive can be expanded to...into a cell line. Where does lentivirus integrate? Genome-wide studies of viral integration have shown that...

  14. CRISPR Plasmids - Epigenetics

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    Collection
    ... Epigenetics Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  16. CRISPR Plasmids - RNA Editing

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    ... RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  18. MAPK Plasmids

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    Collection
    ...Plasmids You may also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated...
Showing: 481 - 500 of 533 results