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We narrowed to 971 results for: lars

Showing: 481 - 500 of 971 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...continues to work on further improvements. In particular, they’re focused on generating variants with ...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic system described above...Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for rapid combinatorial mammalian... genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284. PubMed PMID: 25917172.... 11. Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays...

  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At... tissues. sdAbs can also be used to manipulate cellular functions, like in this early example we highlighted...

  4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...

  5. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...researchers and ready access to stably archived large-scale datasets by computational biologists. ...genome-wide datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it ... interest. In addition, users interested in a particular disease, cell type or drug treatment can browse...used to search the entire CRISPR database for particular screens of interest.   Figure 3: Each ...

  6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...Problems with Chip-seq ChIP-seq typically requires a large amount of input material, cells or tissue, to produce...with cell culture, because it is easy to harvest large quantities of cells. However, when working on specific...false-positives, as proteins may appear to be binding at particular genomic sites when in fact they are just non-...situations when it is technically challenging to collect large numbers of cells or when a high degree of signal...

  7. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...Plasmids with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources ...paper and then another figure in another paper. A large fraction of readers cover the same ground again ...allows you to make meaningful collections from a large section of open access life sciences data. You can...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...easy to navigate. We also updated some our most popular CRISPR blogs to keep them current. Here are some...plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid pages...mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested...only the specified RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular genome...polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system that scientists...translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and ...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...specific to denatured versions, or even to just that particular assay.      Fig. 1: A native protein ...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...talk about Z DNA that inspired her to major in molecular biology in college. She then pursued a PhD in ...not only got incredibly compelled by wet bench molecular research, but also became passionate about virology...of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family... Gene Therapy Center, Associate Director of the Ocular Genomics Institute, and an Associate Member at ...

  13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast." ...measuring autophagy." International journal of molecular sciences 18.9 (2017): 1865.  PubMed PMID: 28846632...

  14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...Twitter if you missed our live updates! My most popular tweet from the conference is shown above. The blog..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...

  15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... by Torsten Wittmann’s lab at UCSF to modulate cellular dynamics with light by modifying EB1. EB1 normally... opens novel ways in manipulating and studying cellular dynamics. The photoinducible control of protein...potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature Cell Biology...the base editing podcast segment The increasing popularity of single base-editors has resulted in the rapid...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic inheritance...began to study other extrachromosomal particles, particularly Resistance or R-factors. Like F-factors, R-factors...especially lambda, was the tool of choice for molecular biologists wanting to study bacterial genetics...pSC101 became the first plasmid cloning vector and molecular biology was never the same. Over the next few ...

  17. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...meetings or calls regularly and don’t cancel, so that you drive progress with regular accountability checks...Mentoring for Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start...Relevant, and Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after ...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    ...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures, and in the presence...

  19. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We ...at the MIT Sloan School of Management. She is particularly interested in exploring how innovative tools...

  20. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...often have to choose the best serotype for their particular study, which includes comparing the transduction...samples are available in various serotypes of two popular control AAV encoding GFP. Check out our inventory...variant provides improved detection for tracking large populations of neurons in wide-field imaging (Dana...on availability date or to be notified when a particular catalog item is available for order please email...
Showing: 481 - 500 of 971 results